■2021年,越南爆发了块状皮肤病(LSD),感染了207,687头牛和水牛,据官方报道,并导致29,182只动物被扑杀。
■在这项研究中,世界动物卫生组织(WOAH)推荐的三种方法证实了越南河廷省死亡并显示典型LSD迹象的牛的样本,并进一步研究了越南和中国的参考菌株与新的临床病例进行比较。
■WOAH推荐的三种试剂检测方法(PCR,病毒分离,和透射电子显微镜)用于确认此临床LSD病例。越南已利用三个众所周知的标记(P32,RPO30和GPCR基因)的序列分析来更好地了解这种流行的病原体。
■我们的发现表明,CX01LSDV菌株与基于P32和RPO30基因的越南参考菌株HL01和中国参考菌株100%相同。有趣的是,GPCR基因的核苷酸序列分析表明,CX01菌株与参考菌株属于同一簇,但它的分支与HL01和中国LSDV菌株不同。CX01株和这些参考病毒株之间的核苷酸鉴定排名99.65%-99.91%,这表明它是LSDV的一种新变体。
■这一发现是新的,表明根据对GPCR基因的分析,至少有两种LSD病毒变体在越南传播。此外,这些结果表明,GPCR基因的序列分析是对越南流行的LSDV进行亚组的重要工具。
UNASSIGNED: In 2021, Vietnam experienced an outbreak of Lumpy skin disease (LSD), which infected 207,687 cattle and buffaloes, as officially reported, and resulted in the culling of 29,182 animals.
UNASSIGNED: In this study, samples from cattle that died and showed typical signs of LSD in the Ha Tinh province of Vietnam were confirmed by three World Organization for Animal Health (WOAH)-recommended methods and further studied to compare the Vietnam and China reference strains to the new clinical cases.
UNASSIGNED: Three methods recommended by WOAH for agent detection (PCR, virus isolation, and transmission electron microscopy) were used to confirm this clinical LSD case. The sequence analysis of three well-known markers (P32, RPO30, and GPCR genes) has been utilized in Vietnam to understand this circulating pathogen better.
UNASSIGNED: Our findings showed that the CX01 LSDV strain is 100% identical to the Vietnam reference strain HL01 and China reference strains based on P32 and RPO30 genes. Interestingly, analysis of the nucleotide sequence of the GPCR gene showed that the CX01 strain belongs to the same cluster as the reference strains, but it has branches different from those of both the HL01 and China LSDV strains. The nucleotide identification between the CX01 strain and these reference virus strains ranked 99.65%-99.91%, suggesting that it is a new variant of LSDV.
UNASSIGNED: This finding is new and indicates that at least two variants of the LSD virus were circulating in Vietnam based on analysis of the GPCR gene. Additionally, these results suggest that the sequence analysis of the GPCR gene is a great tool for subgrouping LSDV circulating in Vietnam.