外源性补充胍基乙酸可以机械地调节肌肉细胞中的能量分布。本研究旨在探讨补充胍基乙酸对肝脏和乳腺肌肉脂肪沉积的影响,脂质水平,和鸭的脂质代谢相关基因表达。我们将480只42日龄的雌性嘉吉鸭随机分为四组,每组6只,每组20只。对照组饲喂基础日粮,实验组饲喂400、600和800mg/kg(GA400、GA600和GA800)胍基乙酸的基础日粮,分别。与对照组相比,(1)总胆固醇(p=0.0262),甘油三酯(p=0.0357),GA400、GA600和GA800在肝脏中的丙二醛(p=0.0452)含量较低;(2)总胆固醇(p=0.0365),甘油三酯(p=0.0459),在GA400,GA600和GA800中,乳腺肌肉中的丙二醛(p=0.0326)含量降低;(3)肝脏中GA600中的高密度脂蛋白(p=0.0356)和载脂蛋白-A1(p=0.0125)含量增加;(4)乳腺肌肉中的载脂蛋白-A1含量(p=0.0489)在GA600和GA800中的含量较高,在GA600中的胰岛素含量为0.08;(脂肪酸转运蛋白1(p=0.0412),和脂蛋白脂酶(p=0.0235)在肝脏中GA400、GA600和GA800中相对表达较高;(8)胰岛素诱导基因1(p=0.0269),脂肪酸转运蛋白1(p=0.0234),和脂蛋白脂酶(p=0.0425)的相对表达在GA400、GA600和GA800中增加。在这项研究中,600mg/kg胍基乙酸的最佳剂量改善了肝脏和乳房肌肉的脂肪沉积,脂质水平,和鸭的脂质代谢相关基因表达。
Exogenous supplementation of guanidinoacetic acid can mechanistically regulate the energy distribution in muscle cells. This study aimed to investigate the effects of guanidinoacetic acid supplementation on liver and breast muscle fat deposition, lipid levels, and lipid metabolism-related gene expression in ducks. We randomly divided 480 42 days-old female Jiaji ducks into four groups with six replicates and 20 ducks for each replicate. The control group was fed the basal diet, and the experimental groups were fed the basal diet with 400, 600, and 800 mg/kg (GA400, GA600, and GA800) guanidinoacetic acid, respectively. Compared with the control group, (1) the total cholesterol (p = 0.0262), triglycerides (p = 0.0357), malondialdehyde (p = 0.0452) contents were lower in GA400, GA600 and GA800 in the liver; (2) the total cholesterol (p = 0.0365), triglycerides (p = 0.0459), and malondialdehyde (p = 0.0326) contents in breast muscle were decreased in GA400, GA600 and GA800; (3) the high density lipoprotein (p = 0.0356) and apolipoprotein-A1 (p = 0.0125) contents were increased in GA600 in the liver; (4) the apolipoprotein-A1 contents (p = 0.0489) in breast muscle were higher in GA600 and GA800; (5) the lipoprotein lipase contents (p = 0.0325) in the liver were higher in GA600 and GA800; (6) the malate dehydrogenase contents (p = 0.0269) in breast muscle were lower in GA400, GA600, and GA800; (7) the insulin induced gene 1 (p = 0.0326), fatty acid transport protein 1 (p = 0.0412), and lipoprotein lipase (p = 0.0235) relative expression were higher in GA400, GA600, and GA800 in the liver; (8) the insulin induced gene 1 (p = 0.0269), fatty acid transport protein 1 (p = 0.0234), and lipoprotein lipase (p = 0.0425) relative expression were increased in GA400, GA600, and GA800 in breast muscle. In this study, the optimum dosage of 600 mg/kg guanidinoacetic acid improved the liver and breast muscle fat deposition, lipid levels, and lipid metabolism-related gene expression in ducks.