粮食及农业组织估计食品生产链损失17%,必须采用科学和技术方法来解决这一问题,以实现可持续性。工业食品生产废弃物及其增值应用,特别是与各种病原微生物和健康相关的影响尚未得到彻底研究。这项研究探讨了食品生产废物提取物-柠檬皮(LP)的潜力,热水(HT),和咖啡silverskin(CSS)作为生物活性化合物的来源。使用氢-甲醇提取进行提取,产率为LP(482mg/1g)>HT(332mg/1g)>CSS(20mg/1g)。琼脂扩散测定法显示,所有三种提取物对赤霉病欧文氏菌均具有实质性的抗菌活性,大肠杆菌,枯草芽孢杆菌,和阿西马里斯芽孢杆菌.所有提取物均显示出针对革兰氏阳性和革兰氏阴性细菌的活性,显示对致病菌如单核细胞增生李斯特菌有效的最低抑制浓度,金黄色葡萄球菌,副溶血性弧菌,和肠沙门氏菌.总酚含量(TPC,以mgGAE/1g为单位)为100、20和100,HT,LP,分别。ABTS的抗氧化活性表明LP的IC50为3.09、13.09和2.61,HT,CSS,分别。此外,通过DPPH测定进一步证实了提取物的抗氧化活性,在CSS(9.84GAEg-1)和LP(9.77mgGAEg-1)中活性最好,而不是在HT(1.45GAEg-1)中活性最好。未观察到对HaCaT细胞的不良细胞毒性作用。胰腺淀粉酶抑制显示抗糖尿病的潜力,LP显示最高水平(92%)。LC-MS表征确定多酚是CSS中的主要化合物,HT中的异戊二烯化化合物,和LP中的黄烷醇。这些发现暗示了工业中食品生产废物的潜在可持续利用。
The Food and Agricultural Organization estimates a 17% loss in the food production chain, making it imperative to adopt scientific and technological approaches to address this issue for sustainability. Industrial food production waste and its value-added applications, particularly in relation to a wide variety of pathogenic microorganisms and the health-related effects have not been thoroughly investigated. This study explores the potential of food production waste extracts-lemon peel (LP), hot trub (HT), and coffee silverskin (CSS) as sources of bioactive compounds. Extraction was conducted using hydro-methanolic extraction with yields in LP (482 mg/1 g) > HT (332 mg/1 g) > CSS (20 mg/1 g). The agar diffusion assay revealed the substantial antibacterial activity of all three extracts against Erwinia Amylovora, Escherichia coli, Bacillus subtilis, and Bacillus aquimaris. All extracts demonstrated activity against Gram-positive and Gram-negative bacteria, displaying minimum inhibitory concentrations effective against pathogenic bacteria like Listeria monocytogenes, Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella enterica. Total phenolic content (TPC in mg GAE/1g) was 100, 20, and 100 for CSS, HT, and LP, respectively. Antioxidant activity by ABTS indicated IC50 of 3.09, 13.09, and 2.61 for LP, HT, and CSS, respectively. Also, the antioxidant activity of the extracts was further confirmed by DPPH assay with the best activity in CSS (9.84 GAEg-1) and LP (9.77 mg of GAEg-1) rather than in HT (1.45 GAEg-1). No adverse cytotoxic effects on HaCaT cells were observed. Pancreatic amylase inhibition demonstrated antidiabetic potential, with LP showing the highest levels (92%). LC-MS characterization identified polyphenols as the main compounds in CSS, prenylated compounds in HT, and flavanols in LP. The findings imply the potential sustainable use of food production waste in industry.