肉桂(Cinnamomumverum)的内部树皮被广泛用作香料。肉桂植物也是用于医疗目的的精油的有价值的来源。本研究旨在研究硬皮精油(CvEO)的组成、体外抗氧化活性及其对CCl4致大鼠肝肾毒性的保护作用。动物组用CvEO(70或100mg/kg体重)预处理7天或不接受治疗,在第7天,使用单剂量的CCl4诱导氧化应激。CCl4给药后24小时,动物被安乐死。在未经治疗的组中,CCl4诱导血清生化参数增加,并引发肝脏和肾脏的氧化应激。CvEO(100mg/kg)导致CCl4升高的丙氨酸转氨酶水平显着降低,天冬氨酸转氨酶,碱性磷酸酶,γ-谷氨酰转移酶,乳酸脱氢酶,总胆固醇,甘油三酯,低密度脂蛋白,尿素,与未治疗组相比,肌酐和高密度脂蛋白水平升高。此外,与未治疗组相比,在给药CCl4前用70和100mg/kg剂量的CvEO预处理可显著降低肝和肾组织中的硫代巴比妥酸反应性物质和蛋白羰基水平.CvEO以100mg/kg的剂量强烈防止了CCl4给药引起的病理性肝肾病变的形成。总的来说,这项研究表明,给予CvEO具有很高的抑制自由基和减轻CCl4诱导的大鼠肝肾毒性的潜力。
The inner bark of cinnamon (Cinnamomum verum) is widely used as a spice. Cinnamon plants are also a valuable source of essential oil used for medicinal purposes. The present study aimed to investigate the composition and in vitro antioxidant activity of essential oil of C. verum bark (CvEO) and its protective effects in vivo on CCl4-induced hepatic and renal toxicity in rats. Groups of animals were pretreated for 7 days with CvEO (70 or 100 mg/kg body weight) or received no treatment and on day 7 a single dose of CCl4 was used to induce oxidative stress. Twenty-four hours after CCl4 administration, the animals were euthanized. In the untreated group, CCl4 induced an increase in serum biochemical parameters and triggered oxidative stress in both liver and kidneys. CvEO (100 mg/kg) caused significant reductions in CCl4-elevated levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, γ-glutamyl transferase, lactate dehydrogenase, total cholesterol, triglycerides, low-density lipoprotein, urea, and creatinine and increased the level of high-density lipoprotein compared with the untreated group. Moreover, pretreatment with CvEO at doses of 70 and 100 mg/kg before administration of CCl4 produced significant reductions in thiobarbituric acid reactive substances and protein carbonyl levels in liver and kidney tissues compared with the untreated group. The formation of pathological hepatic and kidney lesions induced by the administration of CCl4 was strongly prevented by CvEO at a dose of 100 mg/kg. Overall, this study suggests that administration of CvEO has high potential to quench free radicals and alleviate CCl4-induced hepatorenal toxicity in rats.