研究了球磨塑料炭负载纳米零价铁(nZVI@BMPC)及其与厌氧污泥联合用于2,4,6-三氯苯酚(2,4,6-TCP)微生物脱氯的应用。XRD和FTIR分析证明了铁的零价态组成,BET和SEM分析表明,nZVI在BMPC表面均匀分布。连续添加1000mg/L的乳酸钠和nZVI@BMPC增强了厌氧污泥的收获,并导致4-CP在80天内降解。具有nZVI@BMPC的适应财团将2,4,6-TCP完全降解为CH4和CO2,关键的脱氯途径是通过4-CP脱氯和矿化。nZVI@BMPC对2,4,6-TCP的降解率为0.22/d,大于没有nZVI@BMPC的情况。在nZVI@BMPC和铁还原菌控制的Fe2/Fe3系统中,脱氯效率得到了提高。宏基因组分析结果表明,主要的去氯因子是氯氟菌。,Desulfovibrio,和假单胞菌,可以直接将2,4,6-TCP降解为4-CP,尤其是,氯氟菌可同时用于矿化4-CP。功能基因cpra的相对丰度,acoA,acoB,在存在nZVI@BMPC的情况下,tfdB显着增加。本研究提供了一种新的策略,可以很好地替代可能在地下水修复中的应用。
Ball-milled plastic char supported nano zero-valent iron (nZVI@BMPC) and their application combined with anaerobic sludge for microbial dechlorination of 2,4,6-trichlorophenol (2,4,6-TCP) were investigated. The XRD and FTIR analysis proved composition of zero valent states of iron, and the BET and SEM analysis showed that nZVI was uniformly distributed on the surface of BMPC. Successive addition of 1000 mg/L sodium lactate and nZVI@BMPC enhanced the acclamation of anaerobic sludge and resulted in the degradation of 4-CP within 80 days. The acclimated consortium with nZVI@BMPC completely degraded 2,4,6-TCP into CH4 and CO2, and the key dechlorination route was through 4-CP dechlorinaion and mineralization. The degradation rate of 2,4,6-TCP with nZVI@BMPC was 0.22/d, greater than that without nZVI@BMPC. The dechlorination efficiency was enhanced in the Fe2+/Fe3+ system controlled by nZVI@BMPC and iron-reducing bacteria. Metagenomic analysis result showed that the dominant de-chlorinators were Chloroflexi sp., Desulfovibrio, and Pseudomonas, which could directly degrade 2,4,6-TCP to 4-CP, especially, Chloroflexi bacterium could concurrently be used to mineralize 4-CP. The relative abundance of the functional genes cprA, acoA, acoB, and tfdB increased significantly in the presence of the nZVI@BMPC. This study provides a new strategy can be a good alternative for possible application in groundwater remediation.