Abstract：
A novel facultatively anaerobic and Gram-stain-negative bacterium, designated FJH33T, was isolated from mangrove sediment sampled in Zhangzhou, PR China. Cells of strain FJH33T were rod-shaped or slightly curved-shaped, with widths of 0.3-0.5 µm and lengths of 1.0-3.0 µm. Optimum growth of strain FJH33T occurred in the presence of 3 % NaCl (w/v), at 33 °C and at pH 7.0. Oxidase activity was negative, while catalase activity was positive. Its iron-reducing ability was determined. Based on 16S rRNA gene sequence similarity, strain FJH33T was most closely related to Maribellus luteus XSD2T (95.1 %), followed by Maribellus sediminis Y2-1-60T (95.0 %) and Maribellus maritimus 5E3T (94.9 %). Genome analysis of strains FJH33T and M. luteus XSD2T revealed low genome relatedness, with an average nucleotide identity value of 73.8% and a digital DNA-DNA hybridization value of 19.0%. Phylogenetic trees built from 16S rRNA genes and genome sequences showed that strain FJH33T represents a relatively independent phylogenetic lineage within the genus Maribellus. The major cellular fatty acids (≥10 %) were iso-C15 : 0 and C18 : 1 ω9c. The sole respiratory quinone was MK-7. The polar lipids consisted of phosphatidylethanolamine, diphosphatidylcholine, diphosphatidyglycerol and one unidentified lipid. The DNA G+C content was 41.4 mol%. Based on the integrated results of phylogenetic, physiological, biochemical and chemotaxonomic characterizations, we propose that strain FJH33T represents a novel species of the genus Maribellus, for which the name Maribellus mangrovi sp. nov. is proposed. The type strain is FJH33T (=KCTC 102210T=MCCC 1H01459T).
摘要：
一种新型兼性厌氧和革兰氏染色阴性细菌，指定FJH33T,从漳州红树林沉积物中分离出来，公关中国。菌株FJH33T的细胞呈杆状或略微弯曲，宽度为0.3-0.5µm，长度为1.0-3.0µm。菌株FJH33T的最佳生长发生在3%NaCl(w/v)的存在下,在33°C和pH7.0下。氧化酶活性为阴性，而过氧化氢酶活性为阳性。确定了其铁还原能力。基于16SrRNA基因序列相似性,菌株FJH33T与黄牛XSD2T最密切相关(95.1%)，其次是SediminisY2-1-60T（95.0％）和Maribellusmaritimus5E3T（94.9％）。菌株FJH33T和黄牛XSD2T的基因组分析显示低基因组相关性，平均核苷酸同一性值为73.8%，数字DNA-DNA杂交值为19.0%。由16SrRNA基因和基因组序列构建的系统发育树表明，菌株FJH33T代表了Maribellus属中相对独立的系统发育谱系。主要的细胞脂肪酸（≥10％）是异C15：0和C18：1ω9c。唯一的呼吸醌是MK-7。极性脂质由磷脂酰乙醇胺组成，二磷脂酰胆碱，二磷脂酰甘油和一种未鉴定的脂质。DNAG+C含量为41.4mol%。根据系统发育的综合结果，生理,生化和化学分类学特征，我们认为菌株FJH33T代表了Maribellus属的一种新物种，名字为Maribellusmangrovisp。11月。是提议的。菌株类型为FJH33T(=KCTC102210T=MCCC1H01459T)。
