■近年来的一些报道发现肠道菌群与上尿路结石之间存在关联。然而,两者之间的因果关系还有待澄清。
■在孟德尔随机化中,遗传变异被用作推断暴露因素是否对疾病结局有因果影响的工具。我们从MiBioGen联盟发布的肠道微生物组的大型全基因组关联研究中选择了汇总统计数据,样本量为18,340作为暴露因子,并从FinnGenGWAS的上尿路结石数据中选择了4,969例结石病例和213,445例对照作为疾病结果。然后,通过应用逆方差加权,进行了双样本孟德尔随机化分析,MR-Egger,最大似然,和加权中位数。此外,通过敏感性分析排除异质性和水平多效性.
■IVW结果证实了Deltaproteobacteria类(OR=0.814,95%CI:0.666-0.995,P=0.045),NB1n阶(OR=0.833,95%CI:0.737-0.940,P=3.15×10-3),梭菌属1(OR=0.729,95%CI:0.581-0.916,P=6.61×10-3),杆菌属(OR=0.695,95%CI:0.551-0.877,P=2.20×10-3),梭菌属(OR=0.777,95%CI:0.612-0.986,P=0.0380),Flavonifractor属(OR=0.711,95%CI:0.536-0.944,P=0.0181),匈牙利属(OR=0.829,95%CI:0.690-0.995,P=0.0444),和螺旋体属(OR=0.758,95%CI:0.577-0.996,P=0.0464)对上尿路结石有保护作用,而嗜木杆菌(OR=1.26,95%CI:1.010-1.566,P=0.0423)具有相反的作用。敏感性分析未发现异常SNP。
■总之,发现了几个属与上尿路结石之间的因果关系。然而,我们仍需要进一步的随机对照试验来验证.
UNASSIGNED: Several reports in recent years have found an association between gut microbiota and upper urinary urolithiasis. However, the causal relationship between them remains to be clarified.
UNASSIGNED: Genetic variation is used as a tool in Mendelian randomization for inference of whether exposure factors have a causal effect on disease outcomes. We selected summary statistics from a large genome-wide association study of the gut microbiome published by the MiBioGen consortium with a sample size of 18,340 as an exposure factor and upper urinary urolithiasis data from FinnGen GWAS with 4,969 calculi cases and 213,445 controls as a disease outcome. Then, a two-sample Mendelian randomization analysis was performed by applying inverse variance-weighted, MR-Egger, maximum likelihood, and weighted median. In addition, heterogeneity and horizontal pleiotropy were excluded by sensitivity analysis.
UNASSIGNED: IVW results confirmed that class Deltaproteobacteria (OR = 0.814, 95% CI: 0.666-0.995, P = 0.045), order NB1n (OR = 0.833, 95% CI: 0.737-0.940, P = 3.15 × 10-3), family Clostridiaceae1 (OR = 0.729, 95% CI: 0.581-0.916, P = 6.61 × 10-3), genus Barnesiella (OR = 0.695, 95% CI: 0.551-0.877, P = 2.20 × 10-3), genus Clostridium sensu_stricto_1 (OR = 0.777, 95% CI: 0.612-0.986, P = 0.0380), genus Flavonifractor (OR = 0.711, 95% CI: 0.536-0.944, P = 0.0181), genus Hungatella (OR = 0.829, 95% CI: 0.690-0.995, P = 0.0444), and genus Oscillospira (OR = 0.758, 95% CI: 0.577-0.996, P = 0.0464) had a protective effect on upper urinary urolithiasis, while Eubacterium xylanophilum (OR =1.26, 95% CI: 1.010-1.566, P = 0.0423) had the opposite effect. Sensitivity analysis did not find outlier SNPs.
UNASSIGNED: In summary, a causal relationship was found between several genera and upper urinary urolithiasis. However, we still need further randomized controlled trials to validate.