■AFM:芳香指突变体;BH3D:BCL2同源性3结构域;CCD:卷曲螺旋结构域;CD:圆二色谱;[CysDM1]:C18S和C21S双突变体;[CysDM2]:C137S,和C140S双突变体;[CysTM],C18S,C21S,C137S,和C140S四分体突变体;Dmax:最大粒径;dRI,微分折射率;EFA:进化因子分析;FHD:柔性螺旋结构域;FL:全长;GFP:绿色荧光蛋白;HDX-MS:氢/氘交换质谱;ICP-MS:电感耦合等离子体质谱;IDR:固有无序区域;ITC,等温滴定量热法;MALS,多角度光散射;MBP:麦芽糖结合蛋白;MoRFs:分子识别特征;P(r):成对距离分布;PtdIns3K:III类磷脂酰肌醇3-激酶;Rg:回转半径;SASBDB:小角散射生物数据库;SEC:尺寸排阻色谱法;SEC-SAXS:小角散射;TEX-2,TFE:烟草中的尺寸排阻色谱法:2,N,N,N-四(2-吡啶基甲基)-1,2-乙二胺;Vc:相关体积;WT:野生型。
AFM: aromatic finger mutant; BH3D: BCL2 homology 3 domain; CCD: coiled-coil domain; CD: circular dichroism spectroscopy; [CysDM1]: C18S and C21S double mutant; [CysDM2]: C137S, and C140S double mutant; [CysTM], C18S, C21S, C137S, and C140S tetrad mutant; Dmax: maximum particle diameter; dRI, differential refractive index; EFA: evolving factor analysis; FHD: flexible helical domain; FL: full length; GFP: green fluorescent protein; HDX-MS: hydrogen/deuterium exchange mass spectrometry; ICP-MS: inductively coupled plasma mass spectrometry; IDR: intrinsically disordered region; ITC, isothermal titration calorimetry; MALS, multi angle light scattering; MBP: maltose-binding protein; MoRFs: molecular recognition features; P(r): pairwise-distance distribution; PtdIns3K: class III phosphatidylinositol 3-kinase; Rg: radius of gyration; SASBDB: small angle scattering biological data bank; SEC: size-exclusion chromatography; SEC-SAXS: size-exclusion chromatography in tandem with small angle X-ray scattering; TEV: tobacco-etch virus; TFE: 2,2,2-trifluoroethanol; TPEN: N,N,N,N-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine; Vc: volume of correlation; WT: wild-type.