■肝损伤和高脂血症是近年来引起越来越多关注的重大问题。本研究旨在探讨非酰化生长素释放肽(UAG)对小鼠急性肝损伤和高脂血症的影响。
■UAG腹腔注射,每天一次,共三天。上次给药三个小时后,腹腔注射四氯化碳(CCl4)诱导急性肝损伤,分别通过腹腔注射泊洛沙姆407诱导急性高脂血症。二十四小时后,收集样本进行血清生化分析,组织病理学检查,和西方印迹。
■在急性肝损伤小鼠中,UAG显著降低肝脏指数,血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),白细胞介素-6(IL-6),和肿瘤坏死因子-α(TNF-α),肝组织中丙二醛(MDA)浓度降低,超氧化物歧化酶(SOD)升高。肝脏NF-κB(NF-κB)蛋白表达下调。在急性高脂血症小鼠中,UAG显著降低血清总胆固醇(TC),甘油三酯(TG),ALT,AST,以及肝脏TG水平。同时,肝脏MDA显著降低,SOD显著升高。此外,UAG分别改善了CCl4和泊洛沙姆407诱导的肝脏病理损伤。
■腹腔注射UAG对急性肝损伤和高脂血症具有保肝降脂作用,这归因于其抗炎和抗氧化活性。
UNASSIGNED: Liver injury and hyperlipidemia are major issues that have drawn more and more attention in recent years. The present study aimed to investigate the effects of unacylated ghrelin (UAG) on acute liver injury and hyperlipidemia in mice.
UNASSIGNED: UAG was injected intraperitoneally once a day for three days. Three hours after the last administration, acute liver injury was induced by intraperitoneal injection of carbon tetrachloride (CCl4), and acute hyperlipidemia was induced by intraperitoneal injection of poloxamer 407, respectively. Twenty-four hours later, samples were collected for serum biochemistry analysis, histopathological examination, and Western blotting.
UNASSIGNED: In acute liver injury mice, UAG significantly decreased liver index, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), reduced malondialdehyde (MDA) concentration and increased superoxide dismutase(SOD) in liver tissue. NF-kappa B (NF-κB) protein expression in the liver was down-regulated. In acute hyperlipidemia mice, UAG significantly decreased serum total cholesterol (TC), triglyceride (TG), ALT, and AST, as well as hepatic TG levels. Meanwhile, hepatic MDA decreased and SOD increased significantly. Moreover, UAG improved the pathological damage in the liver induced by CCl4 and poloxamer 407, respectively.
UNASSIGNED: Intraperitoneal injection of UAG exhibited hepatoprotective and lipid-lowering effects on acute liver injury and hyperlipidemia, which is attributed to its anti-inflammatory and anti-oxidant activities.