Abstract：
Aim: Animal models of fatal pneumonia caused by Streptococcus pneumoniae (Spn) have not been reliably generated using many strains of less virulent serotypes. Materials & methods: Pulmonary infection of a less virulent Spn serotype1 strain in the immunocompetent mice was established via the intratracheal aerosolization (ITA) route. The survival, local and systemic bacterial spread, pathological changes and inflammatory responses of this model were compared with those of mice challenged via the intratracheal instillation, intranasal instillation and intraperitoneal injection routes. Results: ITA and intratracheal instillation both induced fatal pneumonia; however, ITA resulted in better lung bacterial deposition and distribution, pathological homogeneity and delivery efficiency. Conclusion: ITA is an optimal route for developing animal models of severe pulmonary infections.
What is this article about? Streptococcus pneumoniae (Spn), a type of bacteria, can cause serious illness and death in otherwise healthy people. One way that we study pneumonia is using animals. However, pneumonia in animals infected with Spn in the laboratory does not mimic that in humans very well. To study this illness, we need a new way to set up a proper animal model.What were the results? This study set up a method called intratracheal aerosolization (ITA). In ITA, bacteria can form small droplets called aerosols and reach the deepest parts of a mouse’s lung. ITA can cause deadly illness in mice infected with Spn, even if the mice are healthy.What do the results of the study mean? The ITA method could be a useful tool to set up animal models of serious pneumonia with less virulent bacteria.
What is this article about? Streptococcus pneumoniae (Spn), a type of bacteria, can cause serious illness and death in otherwise healthy people. One way that we study pneumonia is using animals. However, pneumonia in animals infected with Spn in the laboratory does not mimic that in humans very well. To study this illness, we need a new way to set up a proper animal model.What were the results? This study set up a method called intratracheal aerosolization (ITA). In ITA, bacteria can form small droplets called aerosols and reach the deepest parts of a mouse’s lung. ITA can cause deadly illness in mice infected with Spn, even if the mice are healthy.What do the results of the study mean? The ITA method could be a useful tool to set up animal models of serious pneumonia with less virulent bacteria.
摘要：
目的：使用许多毒性较低的血清型菌株，尚未可靠地产生由肺炎链球菌（Spn）引起的致命肺炎的动物模型。材料和方法:通过气管内雾化(ITA)途径在免疫活性小鼠中建立毒性较低的Spn血清型1菌株的肺部感染。生存，局部和全身细菌传播，将该模型的病理变化和炎症反应与通过气管内滴注攻击的小鼠进行比较，鼻内滴注和腹膜内注射途径。结果：ITA和气管内滴注均可引起致命性肺炎；然而，ITA导致更好的肺部细菌沉积和分布，病理同质性和递送效率。结论：ITA是建立重症肺部感染动物模型的最佳途径。
这篇文章是关于什么的？肺炎链球菌（Spn），一种细菌，可能会导致其他健康人群的严重疾病和死亡。我们研究肺炎的一种方法是使用动物。然而,在实验室中感染Spn的动物的肺炎并不能很好地模仿人类的肺炎。为了研究这种疾病，我们需要一种新的方法来建立一个合适的动物模型。结果如何？这项研究建立了一种称为气管内雾化（ITA）的方法。在ITA,细菌可以形成称为气溶胶的小液滴，并到达小鼠肺的最深处。ITA可以在感染Spn的小鼠中引起致命的疾病，即使老鼠是健康的。这项研究的结果意味着什么？ITA方法可能是建立细菌毒性较低的严重肺炎动物模型的有用工具。
这篇文章是关于什么的？肺炎链球菌（Spn），一种细菌，可能会导致其他健康人群的严重疾病和死亡。我们研究肺炎的一种方法是使用动物。然而,在实验室中感染Spn的动物的肺炎并不能很好地模仿人类的肺炎。为了研究这种疾病，我们需要一种新的方法来建立一个合适的动物模型。结果如何？这项研究建立了一种称为气管内雾化（ITA）的方法。在ITA,细菌可以形成称为气溶胶的小液滴，并到达小鼠肺的最深处。ITA可以在感染Spn的小鼠中引起致命的疾病，即使老鼠是健康的。这项研究的结果意味着什么？ITA方法可能是建立细菌毒性较低的严重肺炎动物模型的有用工具。
