可注射骨替代物(IBS)是一种自固定的局部药物递送系统,可调节骨折中骨间隙的形状。这项研究旨在研究牛羟基磷灰石(BHA)和阿仑膦酸盐(Ale)的IBS复合材料在加速骨质疏松大鼠骨骼生长中的有效性。IBS是通过将BHA与5%明胶混合制成的,羟丙基甲基纤维素(HPMC)2%,Ale10%IBS的物理性质是粘度,可注射性,和密度测试。将24只雌性Wistar大鼠分为四组。8周后,在所有大鼠的右股骨中制造2mm间隙并用IBS填充。6周后用X线成像和H、E染色观察愈合过程。获得的结果显示粘度,可注射性,IBS的密度值从30.4到39.4dPa。s,98.22%-98.64%,和0.6325-0.8409g/cm3。X线成像和组织学结果证明了卵巢切除大鼠骨质疏松的状况。BHA-Gel-HPMC-Ale的添加显著影响成骨细胞的数量,骨细胞,破骨细胞(P<0.05)。经过45天的观察,添加BHA-Gel-HPMC-Ale显示成骨细胞的平均数量最高,骨细胞,和破骨细胞,与BHA-Gel-HPMC和阳性对照组相比,分别为25.00±3.00、64.33±11.15和5.67±0.58。BHA-Gel-HPMC-AleIBS具有逆转骨质疏松症的潜力。然而,这些生物材料逆转骨质疏松症的潜在潜力需要进一步研究.
The injectable bone substitute (IBS) is a self-setting local drug delivery system that adjusts the shape of the bone gap in the fracture. This study aimed to examine the effectiveness of IBS composites of bovine hydroxyapatite (BHA) and alendronate (Ale) in accelerating bone growth in osteoporotic rats. IBS was made by mixing BHA with gelatin 5%, hydroxypropyl methylcellulose (HPMC) 2%, and Ale 10%. The physical properties of IBS were viscosity, injectability, and density tests. Twenty-four female Wistar rats were divided into four groups. After 8 weeks, 2 mm gap was made in the right femur of all rats and filled with IBS. The healing process was observed after 6 weeks with X-ray imaging and H and E staining. The obtained results showed viscosity, injectability, and density value of IBS from 30.4 to 39.4 dPa.s, 98.22%-98.64%, and 0.6325-0.8409 g/cm3, respectively. X-ray imaging and histology results proved the condition of osteoporosis in rats with ovariectomy. The addition of BHA-Gel-HPMC-Ale significantly affected the number of osteoblasts, osteocytes, and osteoclasts (P < 0.05). After 45 days of observation, the addition of BHA-Gel-HPMC-Ale showed the highest mean number of osteoblasts, osteocytes, and osteoclasts, which were 25.00 ± 3.00, 64.33 ± 11.15, and 5.67 ± 0.58 compared to BHA-Gel-HPMC and positive control groups. The BHA-Gel-HPMC-Ale IBS has the potential to reverse osteoporosis. Nevertheless, the underlying potential of these biomaterials to reverse osteoporosis needs further research.