PDF(Pubmed)
Abstract：
Demineralized bone matrix (DBM), has been used as a bone-graft material because of its osteoconductivity and osteoinductivity. However, the previous research report that supports the single use of DBM is limited by its rapid resorption caused by the lack of calcium and phosphate. β-Tricalcium phosphate (TCP) is an enriched calcium phosphate material suitable for bone healing with osteoconductive properties. In this study, we have developed injectable bone graft by the loading two kinds of TCP in DBM particles and thermo-sensitive DBM-derived hydrogel (hDBM). TCP powder (pTCP) and TCP granules (gTCP) were loaded into hDBM and DBM, respectively. The bone formation effect was investigated according to the morphological features of TCP. Residual growth factor concentrations were investigated; microstructure and morphology were characterized by SEM. In-vitro studies showed that hDBM/DBM/pTCP and hDBM/DBM/gTCP bone grafts were biocompatible and could promote osteogenesis by up-regulating the expression of Runx2 and OPN, bone-related genes. In-vivo studies using the rabbit-femur defect model revealed that the implanted hDBM/DBM/pTCP bone graft showed similar histology to that of fibrous dysplasia with the expression of CD68, whereas hDBM/DBM/gTCP showed good bone formation. Loading of gTCP in place of pTCP was noticed as an effective way to improve bone regeneration in an injectable hDBM/DBM hydrogel-based bone graft.
摘要：
去矿质骨基质(DBM),由于其骨传导性和骨诱导性，已被用作骨移植材料。然而,先前的研究报告支持单独使用DBM由于缺乏钙和磷酸盐而导致其快速吸收。β-磷酸三钙(TCP)是一种具有骨传导性的适于骨愈合的富集磷酸钙材料。在这项研究中,我们通过在DBM颗粒和热敏DBM衍生的水凝胶（hDBM）中加载两种TCP来开发可注射的骨移植物。将TCP粉末(pTCP)和TCP颗粒(gTCP)装入hDBM和DBM,分别。根据TCP的形态学特征研究骨形成效应。研究了残余生长因子的浓度；通过SEM表征了微观结构和形貌。体外研究表明，hDBM/DBM/pTCP和hDBM/DBM/gTCP骨移植物具有生物相容性，可以通过上调Runx2和OPN的表达促进成骨，骨相关基因.使用兔股骨缺损模型的体内研究表明，植入的hDBM/DBM/pTCP骨移植物显示出与纤维异常增生相似的组织学，并表达CD68，而hDBM/DBM/gTCP显示出良好的骨形成。在可注射的基于hDBM/DBM水凝胶的骨移植物中,负载gTCP代替pTCP被注意为改善骨再生的有效方法。
