革兰氏阴性和杆状细菌,指定C340-1T,从钟山县的稻田土壤中进行了分离和筛选,广西,公关中国。该菌株在20-42°C生长(最佳,37°C),pH5.0-9.0(最佳,pH7.0)和0-4%(w/v)NaCl(最佳,0-1%)在推理者的2A培养基上。该菌株可以固定大气氮和乙炔还原活性,记录到120.26nmol乙烯h-1(mg蛋白)-1。Q-10是唯一的类异戊二烯醌组分;磷脂酰乙醇胺,磷脂酰甘油,磷脂酰胆碱,主要的极性脂质是不明的氨基脂质和不明的极性脂质。求和特征8(C18:1ω7c和/或C18:1ω6c)和求和特征3(C16:1ω7c和/或C16:1ω6c)是主要的细胞脂肪酸。菌株C340-1T的基因组为6.18Mb,G+C含量为69.0摩尔%。基于16SrRNA基因和92个核心基因的系统发育树分析表明,菌株C340-1T与巴西拟螺旋菌JCM1224T型菌株密切相关并成簇,阿根廷固氮螺旋体Az39T,杂螺螺旋藻Sp245T和杂螺螺旋藻JCM17639T。平均核苷酸同一性(ANI),C340-1T菌株与上述密切相关型菌株之间的平均氨基酸同一性(AAI)和数字DNA-DNA杂交(dDDH)值显着低于物种分类的阈值(95-96%,95-96%和70%,分别)。基于系统发育,基因组,表型,生理生化数据,我们有理由相信C340-1T代表了拟螺旋体属的一个新物种,名称为Azospirillumisscasisp。11月。是提议的。菌株类型为C340-1T(=CCTCCAB2023105T=KCTC8126T)。
A Gram-negative and rod-shaped bacterium, designated C340-1T, was isolated and screened from paddy soil in Zhongshan County, Guangxi Province, PR China. This strain grew at 20-42 °C (optimum, 37 °C), pH 5.0-9.0 (optimum, pH 7.0) and 0-4 % (w/v) NaCl (optimum, 0-1 %) on Reasoner\'s 2A medium. The strain could fix atmospheric nitrogen and acetylene reduction activity was recorded up to 120.26 nmol ethylene h-1 (mg protein)-1. Q-10 was the only isoprenoid quinone component; phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and an unidentified polar lipid were the major polar lipids. Summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) were the primary cellular fatty acids. The genome of strain C340-1T was 6.18 Mb, and the G+C content was 69.0 mol%. Phylogenetic tree analysis based on 16S rRNA gene and 92 core genes showed that strain C340-1T was closely related to and clustered with the type strains Azospirillum brasilense JCM 1224T, Azospirillum argentinense Az39T, Azospirillum baldaniorum Sp245T and Azospirillum formosense JCM 17639T. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between strain C340-1T and the closely related type strains mentioned above were significantly lower than the threshold values for species classification (95-96 %, 95-96 % and 70 %, respectively). Based on phylogenetic, genomic, phenotypic, physiological and biochemical data, we have reason to believe that C340-1T represents a new species of the genus Azospirillum, for which the name Azospirillum isscasi sp. nov. is proposed. The type strain is C340-1T(=CCTCC AB 2023105T=KCTC 8126T).