PDF(Pubmed)
Abstract：
Acting as a growth regulator, Indole-3-acetic acid (IAA) is an important phytohormone that can be produced by several Bacillus species. However, few studies have been published on the comprehensive evaluation of the strains for practical applications and the effects of selenium species on their IAA-producing ability. The present study showed the selenite reduction strain Bacillus altitudinis LH18, which is capable of producing selenium nanoparticles (SeNPs) at a high yield in a cost-effective manner. Bio-SeNPs were systematically characterized by using DLS, zeta potential, SEM, and FTIR. The results showed that these bio-SeNPs were small in particle size, homogeneously dispersed, and highly stable. Significantly, the IAA-producing ability of strain was differently affected under different selenium species. The addition of SeNPs and sodium selenite resulted in IAA contents of 221.7 µg/mL and 91.01 µg/mL, respectively, which were 3.23 and 1.33 times higher than that of the control. This study is the first to examine the influence of various selenium species on the IAA-producing capacity of Bacillus spp., providing a theoretical foundation for the enhancement of the IAA-production potential of microorganisms.
摘要：
作为增长调节器，吲哚-3-乙酸（IAA）是一种重要的植物激素，可由几种芽孢杆菌属物种产生。然而,关于实际应用菌株的综合评价以及硒对其IAA生产能力的影响的研究很少。本研究显示了亚硒酸盐还原菌株AltitidinisLH18，它能够以具有成本效益的方式以高产率生产硒纳米颗粒（SeNPs）。使用DLS对Bio-SeNPs进行了系统表征，zeta电位,SEM,和FTIR。结果表明，这些生物SeNPs粒径小，均匀分散，高度稳定。重要的是,不同硒种对菌株产生IAA的影响不同。添加SeNP和亚硒酸钠导致IAA含量为221.7µg/mL和91.01µg/mL，分别,分别是对照组的3.23倍和1.33倍。这项研究是首次研究各种硒对芽孢杆菌产生IAA能力的影响。,为提高微生物产生IAA的潜力提供了理论基础。
