背景:芽孢杆菌属物种合成抗真菌脂肽(LP),使其成为对抗鹰嘴豆枯萎病的可持续和生态友好的管理选择。
结果:在这项研究中,评估了18个内生芽孢杆菌菌株对尖孢镰刀菌的抗真菌活性。与鹰嘴豆枯萎病有关的ciceris(FOC)。其中,13个菌株在直接抗真菌测定中产生显著的抑制区,而5个菌株未能产生FOC的抑制。苏云金芽孢杆菌CHGP12对FOC的抑制作用最高,为3.45cm。从CHGP12中提取的LP显示出对病原体的显著抑制。液相色谱-质谱(LC-MS)分析证实,CHGP12具有产生芬霉素的能力,surfactin,iturin,杆菌烯,杆菌素,植物环孢素,还有杆菌素.在体外定性测定中,CHGP12表现出产生脂肪酶的能力,淀粉酶,纤维素酶,蛋白酶,铁载体,和吲哚3-乙酸(IAA)。使用超高效液相色谱(UPLC)分别用370和770ngmL-1浓度的IAA和赤霉酸(GA)进行定量。此外,与温室实验中的对照相比,CHGP12处理的植物的疾病严重程度比对照降低了40%。此外,CHGP12还表现出植物总生物量的显着增加,即,根和芽生长参数,气孔导度,和光合作用速率。
结论:结论:我们的发现表明,苏云金芽孢杆菌CHGP12是一种有前途的菌株,对鹰嘴豆枯萎病具有很高的拮抗和促进生长的潜力。©2022化学工业学会。
BACKGROUND: Bacillus species synthesize antifungal lipopeptides (LPs) making them a sustainable and eco-friendly management option to combat Fusarium wilt of chickpea.
RESULTS: In this study, 18 endophytic Bacillus strains were assessed for their antifungal activity against Fusarium oxysporum f. sp. ciceris (FOC) associated with Fusarium wilt of chickpea. Among them, 13 strains produced significant inhibition zones in a direct antifungal assay while five strains failed to produce the inhibition of FOC. Bacillus thuringiensis CHGP12 exhibited the highest inhibition 3.45 cm of FOC. The LPs extracted from CHGP12 showed significant inhibition of the pathogen. Liquid chromatography-mass spectrometry (LC-MS) analysis confirmed that CHGP12 possessed the ability to produce fengycin, surfactin, iturin, bacillaene, bacillibactin, plantazolicin, and bacilysin. In an in vitro qualitative assay CHGP12 exhibited the ability to produce lipase, amylase, cellulase, protease, siderophores, and indole 3-acetic acid (IAA). IAA and gibberellic acid (GA) were quantified using ultra-performance liquid chromatography (UPLC) with 370 and 770 ng mL-1 concentrations of IAA and GA respectively. Furthermore, the disease severity showed a 40% decrease over control in CHGP12 treated plants compared to the control in a glasshouse experiment. Moreover, CHGP12 also exhibited a significant increase in total biomass of the plants namely, root and shoot growth parameters, stomatal conductance, and photosynthesis rate.
CONCLUSIONS: In conclusion, our findings suggest that B. thuringiensis CHGP12 is a promising strain with high antagonistic and growth-promoting potential against Fusarium wilt of chickpea. © 2022 Society of Chemical Industry.