关键词: UHPLC-MS antagonism endophytes furanocoumarins in vitro cultures indole 3-acetic acid prenylated flavonoids pterocarpans

来  源:   DOI:10.3389/fpls.2022.1076573   PDF(Pubmed)

Abstract:
UNASSIGNED: Bituminaria bituminosa is a medicinal plant recognized for its phytochemicals, such as furanocoumarins, pterocarpans, and flavonoids. Since the secondary metabolism is influenced by the plant-endophyte interactions, the endophytic bacterial community of B. bituminosa was explored and the possible interactions with the plant were described.
UNASSIGNED: Different bacterial strains were isolated from different organs of in vitro plants as shoots, roots, and seeds. The bacterial strains were identified and phenotypically characterized for different traits; strains were also exposed to different concentrations of B. bituminosa plant extract showing different susceptibility, probably determined by different secondary metabolites produced by the plant in the different organs (i.e. aerial parts and roots).
UNASSIGNED: Bacterial strains showed different phenotypic characteristics; the 6 detected haplotypes were dominated by a single species related to Stenotrophomonas rhizophila. Endophytes isolated from the aerial parts produced a higher indole-3-acetic acid (IAA) amount than those of the roots, while all strains were unable to produce biosurfactants and antagonistic activity toward the other strains. The research opens new perspectives for future analysis addressed to test the susceptibility of the endophytic bacterial community of B. bituminosa toward the pure compounds extracted from the plants, and to investigate the role of these compounds on the distribution of endophytes within the different plant tissues.
摘要:
未经批准:bituminuosabituminosa是一种因其植物化学物质而被认可的药用植物,比如呋喃香豆素,翼果,和类黄酮.由于次生代谢受植物-内生菌相互作用的影响,探索了烟叶的内生细菌群落,并描述了与植物的可能相互作用。
未经鉴定:从离体植物的不同器官中分离出不同的菌株作为芽,根,和种子。鉴定了细菌菌株并对其进行了不同性状的表型表征;还将菌株暴露于显示出不同敏感性的不同浓度的沥青芽孢杆菌植物提取物中。可能是由植物在不同器官(即地上部分和根)中产生的不同次生代谢产物决定的。
UNASSIGNED:细菌菌株表现出不同的表型特征;检测到的6个单倍型主要由与根瘤菌相关的单个物种主导。从地上部分分离出的内生菌产生的吲哚-3-乙酸(IAA)量比根部高,而所有菌株都无法产生生物表面活性剂和对其他菌株的拮抗活性。该研究为未来的分析开辟了新的视角,旨在测试沥青芽孢杆菌的内生细菌群落对从植物中提取的纯化合物的敏感性。并研究这些化合物对不同植物组织内内生菌分布的作用。
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