晚疫病是限制马铃薯产量的主要生物胁迫之一;然而,致病疫霉感染过程的生化机制仍未揭示。在这项研究中,在抗晚疫病马铃薯品种紫云1号(R)和易感品种Favorita(S)上接种了疫霉。非靶向代谢组学用于研究两个品种与病原体在接种后0、48和96小时(hpi)的相容和不相容相互作用中代谢物的变化。共鉴定出819种代谢物,代谢差异主要出现在48hpi后。在相容和不相容的相互作用中,有198和115种差异表达的代谢物(DEM)。这些包括在相容和不相容相互作用期间的147和100个上调的代谢物,分别。其中,73种代谢物被鉴定为致病疫霉响应性DEM。此外,两个品种之间的比较确定了57种与抗性相关的代谢物。抗性马铃薯品种具有较高水平的水杨酸和几种上游苯丙素生物合成代谢产物,三萜类,和羟基肉桂酸及其衍生物,如Sakuranetin,阿魏酸,灵芝酸Mi,葡萄糖酸D2和咖啡酰苹果酸。这些代谢物在细胞壁增厚中起着至关重要的作用,并具有抗菌和抗真菌活性。这项研究报告了马铃薯对致病假单胞菌的时程代谢组学反应。研究结果揭示了马铃薯和致病疫霉的相容和不相容相互作用所涉及的反应。
Late blight is one of the main biological stresses limiting the potato yield; however, the biochemical mechanisms underlying the infection process of Phytophthora infestans remain unrevealed. In this study, the late blight-resistant potato cultivar Ziyun No.1 (R) and the susceptible cultivar Favorita (S) were inoculated with P. infestans. Untargeted metabolomics was used to study the changes of metabolites in the compatible and
incompatible interactions of the two cultivars and the pathogen at 0, 48, and 96 h postinoculation (hpi). A total of 819 metabolites were identified, and the metabolic differences mainly emerged after 48 hpi. There were 198 and 115 differentially expressed metabolites (DEMs) in the compatible and
incompatible interactions. These included 147 and 100 upregulated metabolites during the compatible and
incompatible interactions, respectively. Among them, 73 metabolites were identified as the P. infestans-responsive DEMs. Furthermore, the comparisons between the two cultivars identified 57 resistance-related metabolites. Resistant potato cultivar had higher levels of salicylic acid and several upstream phenylpropanoid biosynthesis metabolites, triterpenoids, and hydroxycinnamic acids and their derivatives, such as sakuranetin, ferulic acid, ganoderic acid Mi, lucidenic acid D2, and caffeoylmalic acid. These metabolites play crucial roles in cell wall thickening and have antibacterial and antifungal activities. This study reports the time-course metabolomic responses of potatoes to P. infestans. The findings reveal the responses involved in the compatible and
incompatible interactions of potatoes and P. infestans.