具有抗生素抗性基因(ARGs)的病原微生物对公共卫生和土壤生态构成严重威胁。虽然新的药物和可用的抗菌材料可以杀死ARG载体,但意外杀死有益的微生物。因此,从土壤中快速富集和分离ARGs及其载体正成为控制ARGs扩散的重要策略。羟肟酸铁载体(HDS)因其参与土壤环境中微生物之间的微量元素转移而受到广泛关注。因此,我们通过荧光假单胞菌HMP01分泌的小分子HDS探索了ARGs及其载体的原位捕获富集方法。在这项研究中,我们证明了HDS显著地原位捕获和富集了某些ARGs,包括氯霉素,MLS,利福霉素,和土壤环境中的四环素抗性基因。富集效率为1473倍,38折,17倍,和5倍,分别,高于对照组。具体来说,初级富集ARGs是rpoB,mphl,catB2和tetA(60),和芽孢杆菌,根瘤菌,Rossellomorea,农杆菌是这些ARGs的宿主。这种富集是由趋化性基因的上调引起的(例如,chew,chec,和cheD)和富集细菌种群内的快速生物膜形成。值得注意的是,代表性的ARG,如猫,macB,rpoB显着降低了36%,85.7%,72%,分别,HDS富集后的水稻土中。我们的研究揭示了铁载体作为敲打剂在土壤环境中生态友好地减少ARGs及其载体的潜在应用。
Pathogenic microorganisms with antibiotic resistance genes (ARGs) pose a serious threat to public health and soil ecology. Although new drugs and available antibacterial materials can kill ARG carriers but accidentally kill beneficial microorganisms. Therefore, the rapid enrichment and separation of ARGs and their carriers from soil is becoming an important strategy for controlling the diffusion of ARGs. Hydroxamate siderophore (HDS) has gained widespread attentions for its involvement in trace element transfer among microorganisms in the soil environment, we thus explored an in-situ trapping-enrichment method for ARGs and their carriers via a small molecular HDS secreted by Pseudomonas fluorescens HMP01. In this study, we demonstrate that HDS significantly in-situ traps and enriches certain ARGs, including chloramphenicol, MLS, rifamycin, and tetracycline resistance genes in the soil environment. The enrichment efficiencies were 1473-fold, 38-fold, 17-fold, and 5-fold, respectively, higher than those in the control group. Specifically, the primary enriched ARGs were rpoB, mphL, catB2, and tetA(60), and Bacillus, Rhizobium, Rossellomorea, and Agrobacterium were hosts for these ARGs. This enrichment was caused by the upregulation of chemotaxis genes (e.g., cheW, cheC, and cheD) and rapid biofilm formation within the enriched bacterial population. Notably, representative ARGs such as cat, macB, and rpoB were significantly reduced by 36%, 85.7%, and 72%, respectively, in the paddy soil after HDS enrichment. Our research sheds light on the potential application of siderophore as a rapping agent for the eco-friendly reduction of ARGs and their carriers in soil environments.