背景:盘旋是一种天然植物,在阿育吠陀中通常被称为Rasayana,主要推荐用于精神刺激和恢复活力治疗。多叶扭转被用作脑补品。据报道,该植物是一种突出的改善记忆的药物。它被用作精神兴奋剂和镇静剂。据报道,它可以减轻精神紧张。
目的:本研究旨在探讨多叶卷叶水醇提取物的保护作用,随着中枢神经系统抑制剂和抗焦虑的活动,在小鼠模型中。
方法:在索氏装置中用水和醇溶液的混合物依次分离从多叶卷叶的提取物。根据OECD准则第2号进行了急性毒性研究。423,其中18只白化病雄性小鼠用不同剂量(1、10、100、500、1000和2000mg/kg)的多叶扭转水醇提取物治疗,并评估毒性参数14天。通过使用各种测试,例如actophotometer,在小鼠中进行了100、200和300mg/kg剂量的多叶卷叶水醇提取物的各种精神运动活动。开放领域,rota-rota,抓地力测试,高架加上迷宫,孔板测试,斜面,烟囱测试。
结果:在急性毒性研究中,从多叶卷叶的水醇提取物被发现属于第4类。因此,选择100、200和300mg/kg剂量的多叶卷叶的水醇提取物用于进一步的药理学研究。精神运动测试的结果(活动光度计,开放领域,rota-rota,握力,孔板测试,斜面,烟囱试验,高架加上迷宫,明暗模型)在小鼠中的测试剂量为100、200和300,显示出CNS抑制作用和抗焦虑作用。
结论:在小鼠模型中,从100、200和300mg/kg剂量的多叶卷叶中获得的水醇提取物显示出中枢神经系统抑制和抗焦虑作用。
BACKGROUND: Convolvulus pluricaulis is a native plant that is commonly mentioned in Ayurveda as a Rasayana and is primarily recommended for use in mental stimulation and rejuvenation therapy. Convolvulus pluricaulis is used as a brain tonic. The plant is reported to be a prominent memory-improving drug. It is used as a psychostimulant and tranquilizer. It is reported to reduce mental tension.
OBJECTIVE: The present study aimed to explore the protective effect of hydroalcoholic extract from the leaves of Convolvulus pluricaulis along with CNS depressant and anti-anxiety activities, in models of mice.
METHODS: The extract from leaves of Convolvulus pluricaulis were sequentially isolated with a mixture of water and alcohol solution in the soxhlet apparatus. An acute toxicity study was conducted as per OECD guidelines no. 423, in which 18 Albino male mice were treated with different doses (1, 10, 100, 500, 1000, and 2000 mg/kg) of hydroalcoholic extract of Convolvulus pluricaulis and assessed for toxicity parameters for 14 days. Various psychomotor activities of hydroalcoholic extract from leaves of Convolvulus pluricaulis for 100, 200, and 300 mg/kg doses were performed in mice by using various tests like actophotometer, open field, rota-rod, grip strength tests, elevated plus maze, hole board test, inclined plane, chimney test.
RESULTS: The hydroalcoholic extract from leaves of Convolvulus pluricaulis was found to fall under category 4 in the acute toxicity study. Therefore, 100, 200, and 300 mg/kg doses of hydroalcoholic extract of leaves of Convolvulus pluricaulis were selected for the further pharmacological study. The results of psychomotor tests (actophotometer, open field, rota-rod, grip strength, hole board test, inclined plane, chimney test, elevated plus maze, light-dark model) for test doses 100, 200, and 300 in mice showed CNS depressant and anti-anxiety effects.
CONCLUSIONS: Hydroalcoholic extract from leaves of Convolvulus pluricaulis at the 100, 200, and 300 mg/kg doses has shown CNS depressant and anti-anxiety effects in mice models.