■在分子水平上了解有机功能对于科学家揭示疾病机制和开发诊断或治疗方法很重要。
■本研究试图找到在11个大鼠器官中选择性表达的基因,包括肾上腺,大脑,结肠,十二指肠,心,回肠,肾,肝脏,肺,脾,脾和胃。
■将三只正常雄性Sprague-Dawley(SD)大鼠麻醉,他们上面提到的器官被收获,并提取新鲜器官中的RNA。使用Solexa高通量测序技术对纯化的RNA进行反转录和测序。基因的丰度通过每百万个碱基对测序的转录物序列的每千碱基片段的期望值(FPKM)来测量。寻找器官中具有最高表达水平的基因,并将其与器官中的中值进行比较。如果最高表达器官中的基因与中等表达器官中的基因显着不同(p<0.05),伴随着q值<0.05,占总丰度的70%以上,该基因被认为是器官中的选择性基因。
■京都基因和基因组百科全书(KEGG),和基因本体论(GO)通路被最高表达的基因富集。根据标准,筛选出1406个选择性基因,其中1,283在基因库中描述,其中123正在等待描述。已知的理解部分证实了器官中的KEGG和GO途径,并且大部分途径需要进一步研究。
■新的选择性基因和有机功能通路有助于科学家在分子水平上揭示器官的机制,和选择性基因\'产物是器官的候选疾病标记。
UNASSIGNED: Understanding organic functions at a molecular level is important for scientists to unveil the disease mechanism and to develop diagnostic or therapeutic methods.
UNASSIGNED: The present study tried to find genes selectively expressed in 11 rat organs, including the adrenal gland, brain, colon, duodenum, heart, ileum, kidney, liver, lung, spleen, and stomach.
UNASSIGNED: Three normal male Sprague-Dawley (SD) rats were anesthetized, their organs mentioned above were harvested, and RNA in the fresh organs was extracted. Purified RNA was reversely transcribed and sequenced using the Solexa high-throughput sequencing technique. The abundance of a gene was measured by the expected value of fragments per kilobase of transcript sequence per million base pairs sequenced (FPKM). Genes in organs with the highest expression level were sought out and compared with their median value in organs. If a gene in the highest expressed organ was significantly different (p < 0.05) from that in the medianly expressed organ, accompanied by q value < 0.05, and accounted for more than 70% of the total abundance, the gene was assumed as the selective gene in the organ.
UNASSIGNED: The Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Ontology (GO) pathways were enriched by the highest expressed genes. Based on the criterion, 1,406 selective genes were screened out, 1,283 of which were described in the gene bank and 123 of which were waiting to be described. KEGG and GO pathways in the organs were partly confirmed by the known understandings and a good portion of the pathways needed further investigation.
UNASSIGNED: The novel selective genes and organic functional pathways are useful for scientists to unveil the mechanisms of the organs at the molecular level, and the selective genes\' products are candidate disease markers for organs.