%0 Journal Article
%T CUT&Tag for Efficient Epigenomic Profiling of Frozen Tissues.
%A Yin Q
%A Li Y
%A Yin Y
%J Methods Mol Biol
%V 2846
%N 0
%D 2024
%M 39141237
暂无%R 10.1007/978-1-0716-4071-5_12
%X Cleavage Under Targets and Tagmentation (CUT&Tag) provides high-resolution sequencing libraries for profiling diverse chromatin components. This protocol details the steps to generate CUT&Tag libraries from fresh or frozen tissues. This CUT&Tag workflow has nine main steps: isolation of nuclei from tissues, binding of nuclei to Concanavalin A-coated beads, binding of the primary antibody, binding of the secondary antibody, binding pA-Tn5 adapter complex, tagmentation, DNA extraction, PCR, and post-PCR cleanup and size selection. This protocol enabled us to generate and sequence CUT&Tag libraries across a broad range of fresh and frozen tissue types.