高静水压力(HHP)处理对冷藏海产品的抗菌作用有据可查,虽然它们对海鲜代谢的影响,尤其是鱼肉的代谢组,并且仍未充分开发。解决这个差距,本研究通过基于非靶向质子核磁共振观察的多变量数据分析,研究了HHP对冷藏玫瑰虾代谢组的影响.真空包装的玫瑰虾样品在0、400、500和600MPa下经受HHP10分钟,然后在2-4°C下储存。在第1天和第14天进行微生物分析和代谢分析。HHP处理可有效灭活乳酸菌。,大肠杆菌,假单胞菌属。,总大肠杆菌,和亚硫酸盐还原厌氧菌.因此,HHP处理显著降低衰变相关代谢物的形成速率,比如次黄嘌呤,三甲胺,和生物胺,在未经处理的样品中表现出显著的积累。多变量无监督分析提供了对HHP诱导的代谢物谱的总体变化的见解。代谢途径分析揭示了腐败的几个潜在途径,包括丙酮酸代谢,缬氨酸,亮氨酸,和异亮氨酸的生物合成,嘌呤代谢,甲烷代谢,甘氨酸,丝氨酸,苏氨酸代谢,柠檬酸盐循环(TCA循环),糖酵解/糖异生,丙氨酸,天冬氨酸,和谷氨酸代谢,硫代谢,泛酸和CoA生物合成,谷胱甘肽代谢,和乙醛酸和二羧酸代谢。重要的是,由于HHP的应用,这些途径发生了改变,特别是在高压水平。总之,结果揭示了HHP对冷藏玫瑰虾影响的潜在机制。
The antimicrobial effects of high hydrostatic pressure (HHP) treatments on chill-stored seafood are well-documented, while their impact on the metabolic profile of seafood, especially the metabolome of fish flesh, and remains underexplored. Addressing this gap, this study investigates the effects of HHP on the metabolome of chill-stored rose shrimp by conducting multivariate data analysis based on untargeted proton nuclear magnetic resonance observations. Vacuum-packed rose shrimp samples were subjected to HHP at 0, 400, 500, and 600 MPa for 10 min and then stored at 2-4°C. The microorganism analysis and metabolic analysis were carried out on days 1 and 14. HHP treatment effectively deactivated Lactobacillus spp., Escherichia coli, Pseudomonas spp., total Coliforms, and sulfite-reducing anaerobic bacteria. Consequently, HHP treatment significantly reduced the formation rate of decay-related metabolites, such as hypoxanthine, trimethylamine, and biogenic amines, which exhibited significant accumulation in untreated samples. Multivariate unsupervised analyses provided insights into the overall changes in the metabolite profile induced by HHP. Metabolic pathway analysis revealed several pathways underlying spoilage, including pyruvate metabolism, valine, leucine, and isoleucine biosynthesis, purine metabolism, methane metabolism, glycine, serine, and threonine metabolism, citrate cycle (TCA cycle), glycolysis/gluconeogenesis, alanine, aspartate, and glutamate metabolism, sulfur metabolism, pantothenate and CoA biosynthesis, glutathione metabolism, and glyoxylate and dicarboxylate metabolism. Importantly, these pathways underwent alterations due to the application of HHP, particularly at high-pressure levels. In summary, the results unveil the potential mechanisms of HHP effects on chill-stored rose shrimps.