农产品中黄曲霉污染的控制方法因其对全球粮食安全的影响而备受关注。我们先前报道了天然谷物挥发性庚醛可以有效抑制黄曲霉的生长,并显示出作为生物防腐剂的巨大潜力。在这项研究中,庚醛的最低抑菌浓度和最低杀菌剂浓度可改变黄曲霉孢子的表面形态,导致它们起皱和塌陷。转录组分析表明,庚烷处理显著改变了几个涉及细胞壁和血浆损伤的基因的表达,活性氧(ROS)积累,能量代谢,AMPK活化蛋白激酶,不饱和脂肪酸的生物合成,RNA降解,和DNA复制。七叶醛诱导黄曲霉孢子早期凋亡的特征是线粒体膜电位降低,增加细胞内ROS的产生,和DNA片段化。这项研究为庚醛对黄曲霉的抑制机制提供了新的见解,并指出了其作为生物防腐剂的潜在应用。关键点:•七叶素可以有效抑制谷物中的黄花生长。•分析了暴露于庚醛的黄曲霉孢子的转录变化。•阐明了庚醛对黄曲霉的抗真菌机制。
Methods of controlling Aspergillus flavus contamination in agro-products have attracted attention because of its impact on global food security. We previously reported that the natural cereal volatile
heptanal could effectively inhibit A. flavus growth and showed great potential as a bio-preservative agent. In this study, the minimum inhibitory concentration and minimum fungicide concentration of
heptanal could change the surface morphology of A. flavus spores, causing them to wrinkle and collapse. Transcriptomic analysis showed that
heptanal treatment significantly changed the expression of several genes involved in cell wall and plasma damage, reactive oxygen species (ROS) accumulation, energy metabolism, AMPK-activated protein kinase, biosynthesis of unsaturated fatty acids, RNA degradation, and DNA replication. Heptanal-induced early apoptosis of A. flavus spores was characterized by decreased mitochondrial membrane potential, increased intracellular ROS production, and DNA fragmentation. This study provides new insight into the inhibitory mechanism of
heptanal against A. flavus and points to its potential application as a bio-preservative. KEY POINTS: • Heptanal can effectively inhibit A. flavus growth in cereal grains. • The transcriptional changes in A. flavus spores exposed to
heptanal were analyzed. • The antifungal mechanism of
heptanal against A. flavus was elucidated.