近年来,非酒精性脂肪性肝病(NAFLD)的患病率急剧上升,它与代谢性疾病高度相关,以及肝细胞癌的发展。然而,治疗NAFLD的有效治疗策略仍然很少.尽管富氢水对肝脂肪变性显示出有益的作用,不便限制了这种抗氧化剂的应用。鉴于此,富氢珊瑚钙(HRCC)是由于其方便和可量化的特性而开发的。然而,HRCC对NAFLD的影响尚不清楚。在本研究中,我们发现HRCC治疗改善蛋氨酸和胆碱缺乏饮食(MCD)诱导的肝脂肪变性,天冬氨酸转氨酶和丙氨酸转氨酶水平升高,小鼠肝脏炎症因子表达升高。除了抗氧化酶的表达增加,我们发现HRCC增加了胆汁酸生物合成相关基因的表达,包括Cyp8b1和Cyp27a1。肝胆汁酸含量增加,比如嗜酸,23去氧胆酸,甘草脱氧胆酸,和胆酸,在用HRCC治疗的MCD小鼠中也得到证实。由于胆汁酸的生物发生与肠道微生物组的构成有关,通过HRCC评估肠道微生物组的变化。我们发现HRCC显著改变了MCD小鼠肠道菌群的构成,增加了厌氧菌的含量,Acutalibacter,厌氧菌,和棒状杆菌。一起来看,HRCC通过抗炎机制和增加抗氧化活性改善MCD诱导的NAFLD。此外,HRCC可能会改变肠道微生物组以改变肝脏胆汁酸含量,为NAFLD的治疗发挥有益作用。
The prevalence of non-alcoholic fatty liver disease (NAFLD) has dramatically increased in recent years, and it is highly associated with metabolic diseases, as well as the development of hepatocellular carcinoma. However, effective therapeutic strategies for the treatment of NAFLD are still scarce. Although hydrogen-rich water shows beneficial effects for hepatic steatosis, the inconvenience limits the application of this antioxidant. In light of this, hydrogen-rich coral calcium (HRCC) was developed due to its convenience and quantifiable characteristics. However, the effects of HRCC on NAFLD are still unknown. In the present study, we found that HRCC treatment improved methionine-and-choline-deficient diet (MCD)-induced hepatic steatosis, increased aspartate aminotransferase and alanine aminotransferase levels, and elevated hepatic inflammatory factor expressions in mice. In addition to the increased expressions of antioxidative enzymes, we found that HRCC increased the expressions of bile acid biosynthesis-related genes, including Cyp8b1 and Cyp27a1. Increased hepatic bile acid contents, such as muricholic acids, 23 nor-deoxycholic acid, glycoursodeoxycholic acid, and cholic acids, were also confirmed in MCD mice treated with HRCC. Since the biogenesis of bile acids is associated with the constitution of gut microbiome, the alterations in gut microbiome by HRCC were evaluated. We found that HRCC significantly changed the constitution of gut microbiome in MCD mice and increased the contents of Anaerobacterium, Acutalibacter, Anaerosacchariphilus, and Corynebacterium. Taken together, HRCC improved MCD-induced NAFLD through anti-inflammatory mechanisms and by increasing antioxidative activities. Additionally, HRCC might alter gut microbiome to change hepatic bile acid contents, exerting beneficial effects for the treatment of NAFLD.