PDF(Pubmed)
Abstract：
Obesity and its associated hepatic steatosis have become a global concern, posing numerous health hazards. Photodynamic therapy (PDT) is a unique approach that promotes anti-obesity by releasing intracellular fat. Chlorin e6 (Ce6)-PDT was tested for its anti-obesity properties in male ovariectomized (OVX) beagle dogs, as well as male C57BL/6 and Balb/c mice. The 12 OVX beagles were randomly assigned to one of four groups: high-fat diet (HFD) only, Ce6 only, Ce6 + 10 min of light-emitting diode light (LED) treatment, and Ce6 + 15 min of light treatment. We assessed several parameters, such as body weight, adipose tissue morphology, serum biochemistry, and body fat content analysis by computed tomography (CT) scan in HFD-fed beagle dogs. At the end of the study period, dogs that were treated for 35 days with Ce6 and exposed to LED irradiation (660 nm) either for 10 min (Ce6 + 10 min of light) or for 15 min (Ce6 + 15 min of light) had decreased body weight, including visceral and subcutaneous fats, lower aspartate transaminase (AST)/alanine transaminase (ALT) ratios, and a reduction in the area of individual adipocytes with a concomitant increase in the number of adipocytes. Furthermore, C57BL/6 male mice following an HFD diet were effectively treated by Ce6-PDT treatment through a reduction in weight gain and fat accumulation. Meanwhile, Ce6-PDT attenuated hepatocyte steatosis by decreasing the epididymal adipose tissue and balloon degeneration in hepatocytes in HFD-fed Balb/c mice. Taken together, our results support the idea that Ce6-PDT is a promising therapeutic strategy for the recovery of obesity and obesity-related hepatic steatosis.
摘要：
肥胖及其相关的肝脏脂肪变性已成为全球关注的问题,带来许多健康危害。光动力疗法(PDT)是通过释放细胞内脂肪来促进抗肥胖的独特方法。在雄性卵巢切除（OVX）比格犬中测试了氯元素e6（Ce6）-PDT的抗肥胖特性，以及雄性C57BL/6和Balb/c小鼠。12只OVX小猎犬被随机分配到四组中的一组：仅高脂饮食（HFD），仅限Ce6,Ce6+10分钟的发光二极管灯（LED）处理，和Ce6+15分钟的光处理。我们评估了几个参数，比如体重，脂肪组织形态学,血清生物化学,通过HFD喂养的比格犬的计算机断层扫描（CT）扫描进行体脂含量分析。在研究期结束时，用Ce6治疗35天并暴露于LED照射（660nm）10分钟（Ce6+10分钟的光）或15分钟（Ce6+15分钟的光）的狗体重减轻，包括内脏和皮下脂肪，降低天冬氨酸转氨酶（AST）/丙氨酸转氨酶（ALT）比率，和单个脂肪细胞面积的减少，同时脂肪细胞数量的增加。此外，通过减少体重增加和脂肪积累,通过Ce6-PDT处理有效地处理HFD饮食后的C57BL/6雄性小鼠。同时,Ce6-PDT通过减少HFD喂养的Balb/c小鼠的附睾脂肪组织和肝细胞中的球囊变性来减轻肝细胞脂肪变性。一起来看,我们的结果支持这样一种观点,即Ce6-PDT是一种有前景的治疗肥胖和肥胖相关性肝脂肪变性的治疗策略.
