这项研究的目的是检查微生物特征,产生的代谢物,以及位于Keana的采矿土壤中存在的潜在放射性风险,尼日利亚中北部。从Keana的各个地方收集了土壤样本,纳萨拉瓦州。进行了细菌分离,和分子技术被用来表征在收集的土壤样品中发现的细菌。此外,确定了这些分离株对抗生素的敏感性,和细菌筛选它们产生代谢产物的能力。分离的细菌分为三类:放线菌,Firmicutes,和变形杆菌。对光谱的分析表明,产生了1595种化合物,包括羧酸,硝基化合物,醛类,酸酐,酯类,酮,酰胺,酚类物质,酒精,烷烃,烯烃,炔烃,和芳烃。产生的一些代谢物是油酸,1,3-二氧戊环,亚油酸和油酸,1-壬烯,丁基化羟基甲苯,邻苯二甲酸二异辛酯,邻苯二甲酸二(2-乙基己基)酯,和1,2-苯二羧酸(85.32%)是产生最多的代谢产物。在测试的抗生素中,左氧氟沙星和环丙沙星对分离物具有最强的抗菌性能。机载伽马射线光谱分析确定钾水平升高,钍,土壤中的铀,表明潜在的环境危害。然而,细菌和放射性元素之间没有发现显著的相关性。这些发现强调了在Keana进行全面环境监测以解决与微生物污染和放射性物质相关的潜在健康风险的重要性。此外,该研究强调了Keana土壤中微生物多样性在促进次生代谢产物生产中的作用,并具有在制药和工业部门的潜在应用。.
The objective of the research was to examine microbial characteristics, metabolites produced, and the potential radiological risks present in mining soils located in Keana, North Central Nigeria. Soil samples were collected from various locations within Keana, Nasarawa State. Bacterial isolation was carried out, and molecular techniques were employed to characterize the bacteria found in the collected soil samples. Additionally, the susceptibility of these isolates to antibiotics was determined, and the bacteria screened for their ability to produce metabolites. The isolated bacteria were classified into three groups: Actinobacteria, Firmicutes, and Proteobacteria. The analysis of the spectra revealed that 1595 compounds were produced, including carboxylic acids, nitro compounds, aldehydes, anhydrides, esters, ketones, amides, phenols, alcohols, alkanes, alkenes, alkynes, and arenes. Some of the metabolites produced were oleic acid, 1,3-dioxolane, linoelaidic acid and oleic acid, 1-nonadecene, butylated hydroxytoluene, diisooctyl phthalate, bis(2-ethylhexyl) phthalate among others, and 1,2-benzenedicarboxylic acid (85.32%) as the most produced metabolite. Among the antibiotics tested, levofloxacin and ciprofloxacin exhibited the strongest antibacterial properties against the isolates. Airborne gamma-ray spectrometry analysis identified elevated levels of potassium, thorium, and uranium in the soils, indicating potential environmental hazards. However, no significant correlation was found between the presence of bacteria and radioactive elements. These findings emphasize the importance of comprehensive environmental monitoring in Keana to address potential health risks associated with microbial contamination and radioactive materials. Additionally, the study highlighted the role of microbial diversity in Keana soils in promoting the production of secondary metabolites with potential applications in pharmaceutical and industrial sectors..