昆虫具有复杂和动态的肠道微生物系统,这有助于宿主营养吸收,繁殖,能量代谢,和防止压力。然而,关于宿主-肠道细菌微生物群通过miRNA(microRNA)调节的相互作用的数据有限,Riptortuspedestris.这里,我们进行了16SrRNA扩增子测序和小RNA测序从R.pedestris肠道在三种环境条件和抗生素处理,这表明我们通过汇编获得了大量的读段,过滤和质量控制。16SrRNA扩增子测序结果表明,抗生素治疗组与其他组之间肠道菌群的丰度和多样性发生了显著变化,基于功能预测,它们参与代谢和生物合成相关功能。此外,我们基于高质量的可映射读段鉴定了不同数量的差异表达的单基因(DEG)和差异表达的miRNA(DEM),它们富含各种免疫相关的途径,包括Toll样受体,RIG-I样受体,NOD样受体,JAK/STAT,PI3K/Akt,NF-κB,MAPK信号通路,等等,使用GO和KEGG富集分析。稍后,预测并随机选择鉴定出的miRNAs及其靶基因以构建相互作用网络。最后,我们的研究表明,肠道细菌菌群的改变与Toll/Imd信号通路的DEM呈显著正相关或负相关。一起来看,我们的研究结果为进一步深入理解肠道菌群与通过miRNA调控的免疫反应之间的相互作用奠定了基础,为半翅目害虫的防治提供新的依据。
Insects possess complex and dynamic gut microbial system, which contributes to host nutrient absorption, reproduction, energy metabolism, and protection against stress. However, there are limited data on interactions of host-gut bacterial microbiota through miRNA (microRNA) regulation in a significant pest, Riptortus pedestris. Here, we performed the 16S rRNA amplicon sequencing and small RNA sequencing from the R. pedestris gut under three environmental conditions and antibiotic treatment, suggesting that we obtained a large amount of reads by assembly, filtration and quality control. The 16S rRNA amplicon sequencing results showed that the abundance and diversity of gut bacterial microbiota were significantly changed between antibiotic treatment and other groups, and they are involved in metabolism and biosynthesis-related function based on functional prediction. Furthermore, we identified different numbers of differentially expressed unigenes (DEGs) and differentially expressed miRNAs (DEMs) based on high-quality mappable reads, which were enriched in various immune-related pathways, including Toll-like receptor, RIG-I-like receptor, NOD-like receptor, JAK/STAT, PI3K/Akt, NF-κB, MAPK signaling pathways, and so forth, using GO and KEGG enrichment analysis. Later on, the identified miRNAs and their target genes in the R. pedestris gut were predicted and randomly selected to construct an interaction network. Finally, our study indicated that alterations in the gut bacterial microbiota are significantly positively or negatively associated with DEMs of the Toll/Imd signaling pathway with Pearson correlation analysis. Taken together, the results of our study lay the foundation for further deeply understanding the interactions between the gut microbiota and immune responses in R. pedestris through miRNA regulation, and provide the new basis for pest management in hemipteran pests.