PDF(Pubmed)
Abstract：
Insects depend on humoral immunity against intruders through the secretion of antimicrobial peptides (AMPs) and immune effectors via NF-κB transcription factors, and their fitness is improved by gut bacterial microbiota. Although there are growing numbers of reports on noncoding RNAs (ncRNAs) involving in immune responses against pathogens, comprehensive studies of ncRNA-AMP regulatory networks in Riptortus pedestris, which is one of the widely distributed pests in East Asia, are still not well understood under feeding environmental changes. The objective of this study employed the whole-transcriptome sequencing (WTS) to systematically identify the lncRNAs (long noncoding RNA) and circRNAs (circular RNA) and to obtain their differential expression from the R. pedestris gut under different feeding conditions. Functional annotation indicated that they were mainly enriched in various biological processes with the GO and KEGG databases, especially in immune signaling pathways. Five defensin (four novel members) and eleven lysozyme (nine novel members) family genes were identified and characterized from WTS data, and meanwhile, phylogenetic analysis confirmed their classification. Subsequently, the miRNA-mRNA interaction network of above two AMPs and lncRNA-involved ceRNA (competing endogenous RNA) regulatory network of one lysozyme were predicted and built based on bioinformatic prediction and calculation, and the expression patterns of differentially expressed (DE) defensins, and DE lysozymes and related DE ncRNAs were estimated and selected among all the comparison groups. Finally, to integrate the analyses of WTS and previous 16S rRNA amplicon sequencing, we conducted the Pearson correlation analysis to reveal the significantly positive or negative correlation between above DE AMPs and ncRNAs, as well as most changes in the gut bacterial microbiota at the genus level of R. pedestris. Taken together, the present observations provide great insights into the ncRNA regulatory networks of AMPs in response to rearing environmental changes in insects and uncover new potential strategies for pest control in the future.
摘要：
昆虫通过分泌抗菌肽（AMPs）和通过NF-κB转录因子的免疫效应因子来依赖针对入侵者的体液免疫，肠道细菌菌群改善了它们的适应性。尽管有越来越多的非编码RNA（ncRNA）参与针对病原体的免疫应答的报道，Riptortuspedestris中ncRNA-AMP调控网络的综合研究，这是东亚广泛分布的害虫之一，在喂养环境变化的情况下，仍然没有得到很好的理解。这项研究的目的是采用全转录组测序（WTS）来系统地鉴定lncRNAs（长链非编码RNA）和circRNAs（环状RNA），并在不同的喂养条件下获得它们从牛腹中的差异表达。功能注释表明，它们主要富集在GO和KEGG数据库的各种生物过程中，尤其是在免疫信号通路中。从WTS数据中鉴定并表征了5个防御素（4个新成员）和11个溶菌酶（9个新成员）家族基因，同时,系统发育分析证实了它们的分类。随后，基于生物信息学预测和计算，预测并建立了上述两种AMPs的miRNA-mRNA相互作用网络和一种溶菌酶的lncRNA参与的ceRNA（竞争性内源性RNA）调控网络，和差异表达（DE）防御素的表达模式，在所有比较组中估计和选择DE溶菌酶和相关的DEncRNAs。最后,整合WTS和之前的16SrRNA扩增子测序的分析，我们进行了Pearson相关性分析，以揭示上述DEAMP和ncRNAs之间的显著正相关或负相关，以及R.pedestris属水平的肠道细菌菌群的大多数变化。一起来看,当前的观察结果为响应昆虫饲养环境变化的AMP的ncRNA调控网络提供了很好的见解，并揭示了未来害虫防治的新潜在策略。
