鹅星状病毒(GAstV)是一种小型,无包裹,单股,正义性RNA病毒。自2016年以来,GAstV迅速在中国各个地区传播。在四川,在2019年至2022年期间从鹅病中收集的113个样本中,有97个通过PCR检测对GAstV呈阳性。值得注意的是,在过去的三年里,四川的GAstV爆发占所有鹅源病毒的85.8%。在这些案例中,63.9%有单一的GAstV感染,29.9%有双重感染,和6.2%有四重感染。了解GAstV不同毒株之间的毒力变化。分离出12个代表性的单一GAstV感染菌株。这些菌株表现出独特的特征,如器官和关节中突出的白色尿酸盐沉积,以及主要靶器官组织中广泛的组织吞噬作用。对这些代表性的GAstV毒株的保守ORF1b基因和可变ORF2基因进行测序,能够为GAstV建立系统发育树。所有GAstV菌株都被鉴定为属于具有不同内部基因序列的基因型2。在体内和体外对GAstV基因型2进行了实验,揭示了易感细胞间致病性和毒力的显著差异,胚胎,还有小鹅.这项全面的研究增强了研究人员对GAstV基因型2的传播特征和毒力的理解,有助于更好地理解其分子流行病学和致病机制。
Goose astrovirus (GAstV) is a small, non-enveloped, single-stranded, positive-sense RNA virus. GAstV has rapidly spread across various regions in China since 2016. In Sichuan, out of 113 samples were collected from goose diseases between 2019 and 2022, 97 were positive for GAstV through PCR testing. Remarkably, over the past three years, GAstV outbreak in Sichuan has accounted for an astonishing 85.8% of all goose-origin viruses. Among these cases, 63.9% had single GAstV infections, 29.9% had dual infections, and 6.2% had quadruple infections. To comprehend the variations in virulence among distinct strains of GAstV. 12 representative strains of single GAstV infections were isolated. These strains exhibited distinct characteristics, such as prominent white urate depositions in organs and joints, as well as extensive tissues phagocytosis in major target organs\' tissues. The conserved ORF1b genes and the variable ORF2 genes of these representative GAstV strains were sequenced, enabling the establishment of phylogenetic trees for GAstV. All GAstV strains were identified as belonging to genotype-2 with varying internal gene sequences. Experiments were conducted on GAstV genotype-2, both in vivo and in vitro, revealed significant variations in pathogenicity and virulence across susceptible cells, embryos, and goslings. This comprehensive study enhances researchers\' understanding of the transmission characteristics and virulence of GAstV genotype-2, aiding in a better comprehension of their molecular epidemiology and pathogenic mechanism.