Ipomoea(Convolvulaceae)的乳胶是一种特殊的酰基糖的来源,称为树脂糖苷,因其生物活性而受到高度赞赏(即泻药,抗菌,细胞毒性等。).大多数研究是在块根多年生植物中进行的,储存树脂糖苷的地方。然而,它们的含量和变异在缺乏这种根的一年生藤本植物中是未知的,例如在Ipomoea寄生的情况下。该物种通过其快速生长包含研究/生物学和人类价值,在恶劣的环境中生存,以及在人类中就业以改善心理/认知能力。这些特性使I.parasitica成为一种理想的系统,以分析树脂糖苷及其响应于edaphocliate的变化。在墨西哥中部的两个地方收集了I.parasitica叶柄的表土样品(0-30厘米深)和乳胶。通过UHPLC-ESI-QTOF分析胶乳,和土壤理化特性,降雨,minimum,平均,并记录了最高温度。我们还测量了冠层(%),岩石(%),和植物覆盖率(%)。进行了主成分分析,以发现edaphocliate和树脂糖苷之间的关联。在I.parasitica的胶乳中发现了44种树脂糖苷。10个与三组分显著相关(47.07%),含有四糖,五糖,和四糖单元的二聚体。五种树脂糖苷被认为是组成型的,因为它们在所有植物中。然而,每个地方都存在独特的分子,我们假设这是对本研究中发现的重要微生境条件(温度,粘土含量,pH值,和钾)。我们的结果表明,在I.parasitica胶乳中存在树脂糖苷,并且是实验测试上述条件对这些分子的影响的基础。然而,生态,分子,和生化因素应该在设计来产生这些复杂分子的实验中考虑。
The latex of Ipomoea (Convolvulaceae) is a source of a special kind of acylsugars called resin
glycosides, which are highly appreciated because of their biological activities (i.e. laxative, antimicrobial, cytotoxic etc.). Most research has been conducted in perennials with tuberous roots, where resin
glycosides are stored. However, their content and variation are unknown in annual vines that lack this type of root, such as in the case of Ipomoea parasitica. This species contains research/biological and human value through its fast growth, survival in harsh environments, and employment in humans for mental/cognitive improvements. These qualities make I. parasitica an ideal system to profile resin
glycosides and their variations in response to edaphoclimate. Topsoil samples (0-30 cm depth) and latex from petioles of I. parasitica were collected in two localities of central Mexico. The latex was analyzed through UHPLC-ESI-QTOF, and soil physico-chemical characteristics, the rainfall, minimum, average, and maximum temperatures were recorded. We also measured canopy (%), rockiness (%), and plant cover (%). A Principal Component Analysis was conducted to find associations between edaphoclimate and the resin glycosides. Forty-four resin
glycosides were found in the latex of I. parasitica. Ten correlated significantly with three components (47.07%) and contained tetrasaccharide, pentasaccharide, and dimers of tetrasaccharide units. Five resin
glycosides were considered constitutive because they were in all the plants. However, exclusive molecules to each locality were also present, which we hypothesize is in response to significant microhabitat conditions found in this study (temperature, clay content, pH, and potassium). Our results showed the presence of resin glycosides in I. parasitica latex and are the basis for experimentally testing the effect of the conditions above on these molecules. However, ecological, molecular, and biochemical factors should be considered in experiments designed to produce these complex molecules.