目的:本研究旨在研究熊果苷在三硝基苯磺酸(TNBS)诱导的实验性结肠炎模型中可能的积极作用,为了与美沙拉嗪进行比较,用于治疗炎症性肠病,并观察其伴随使用的效果。
方法:将40只Wistar白化病雄性大鼠随机分为5组,作为对照组。结肠炎,结肠炎+熊果苷(Arb),结肠炎+美沙拉嗪(Mes),结肠炎+美沙拉嗪+熊果苷(M+A)。促炎细胞因子[白细胞介素(IL)-6,IL-1β,肿瘤坏死因子α(TNF-α)]和氧化剂/抗氧化剂参数[丙二醛(MDA),超氧化物歧化酶抑制(SOD)抑制,髓过氧化物酶(MPO),和过氧化氢酶,从样品中处理谷胱甘肽过氧化物酶(GPx)]。组织病理学评估评估杯状细胞减少,细胞浸润,粘膜丢失.
结果:当比较治疗组和TNBS组时,MDA具有统计学意义,MPO,SOD抑制,GPx值,IL-6、IL-1β和TNF-α水平。组织病理学评估显示,与TNBS组相比,一起使用美沙拉嗪和熊果苷的组的粘膜损失值在统计学上显着降低。
结论:我们的研究结果阐明,单独使用熊果苷或与美沙拉嗪联合使用对结肠炎诱导的大鼠产生积极作用。
OBJECTIVE: This study aimed to investigate the possible positive effects of arbutin in a trinitrobenzene sulfonic acid (TNBS)- induced experimental colitis model, to compare it with mesalazine, which is used in treating inflammatory bowel disease and to observe the effect of its concomitant use.
METHODS: Forty Wistar albino species male rats were randomized into 5 groups as control, colitis, colitis+arbutin (Arb), colitis+mesalazine (Mes), and colitis+mesalazine+arbutin (M+A). Proinflammatory cytokines [interleukin (IL)-6, IL-1β, tumor necrosis factor alpha (TNF-α)] and oxidant/antioxidant parameters [malondialdehyde (MDA), superoxide dismutase inhibition (SOD) inhibition, myeloperoxidase (MPO), and catalase, glutathione peroxidase (GPx)] were processed from the samples. Histopathological evaluation evaluated goblet cell reduction, cellular infiltration, and mucosal loss.
RESULTS: When the treatment groups and the TNBS group were compared, statistical significance was achieved in MDA, MPO, SOD inhibition, GPx values, IL-6, IL-1β and TNF-α levels. Histopathological evaluation revealed a statistically significant decrease in the mucosal loss value in the group where mesalazine and arbutin were used together compared to the TNBS group.
CONCLUSIONS: Our study\'s results elaborated that using arbutin alone or in combination with mesalazine produced positive effects in colitis-induced rats.