■萝卜籽是一种功能性食品,具有许多有益的健康作用。芥子油苷是萝卜种子中的特征性成分,可以通过肠道菌群转化为生物活性异硫氰酸盐。
■本研究旨在评估萝卜籽芥子油苷(RSG)的抗肥胖功效,并探索其潜在机制,重点关注肠道微生物群和粪便代谢组。
■向高脂肪饮食诱导的肥胖小鼠补充不同剂量的RSG提取物8周。体重的变化,血脂,丙氨酸氨基转移酶(ALT),和天冬氨酸氨基转移酶(AST)水平;并检查肝脏和脂肪组织的病理变化。粪便代谢组和16SrRNA基因测序用于分析粪便代谢产物丰度和肠道微生物群的变化。分别。
■结果表明,RSG提取物可防止体重增加并降低血脂,ALT,肥胖小鼠肝脏和附睾脂肪细胞中的AST水平和脂质沉积。用RSG提取物治疗还增加了肠道微生物群的多样性,并改变了肠道微生物群中的优势菌属,降低粪杆菌的丰度,增加Allobaculum的丰度,Romboutsia,Turicibacter,还有Akkermansia.粪便代谢组结果鉴定出570种差异丰富的代谢物,其中芥子油苷降解产物,如莱芬和7-甲基亚磺酰基庚基异硫氰酸酯,RSG提取物干预后显著上调。此外,代谢途径的富集分析表明,RSG提取物的抗肥胖作用可能是由胆汁分泌的改变介导的,脂肪的消化吸收,和植物次生代谢产物的生物合成。总的来说,RSG提取物可以抑制肥胖的发展,RSG的肥胖缓解作用与肠道菌群和芥子油苷代谢物的替代调节有关。
UNASSIGNED: Radish seed is a functional food with many beneficial health effects. Glucosinolates are characteristic components in radish seed that can be transformed into bioactive isothiocyanates by gut microbiota.
UNASSIGNED: The present study aims to assess anti-obesity efficacy of radish seed glucosinolates (RSGs) and explored the underlying mechanisms with a focus on gut microbiota and fecal metabolome.
UNASSIGNED: High-fat diet-induced obese mice were supplemented with different doses of RSGs extract for 8 weeks. Changes in body weight, serum lipid, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels; and pathological changes in the liver and adipose tissue were examined. Fecal metabolome and 16S rRNA gene sequencing were used to analyze alterations in fecal metabolite abundance and the gut microbiota, respectively.
UNASSIGNED: Results showed that RSG extract prevented weight gain and decreased serum lipid, ALT, AST levels and lipid deposition in liver and epididymal adipocytes in obese mice. Treatment with RSG extract also increased gut microbiota diversity and altered the dominant bacteria genera in the gut microbiota, decreasing the abundance of Faecalibaculum and increasing the abundance of Allobaculum, Romboutsia, Turicibacter, and Akkermansia. Fecal metabolome results identified 570 differentially abundant metabolites, of which
glucosinolate degradation products, such as sulforaphene and 7-methylsulfinylheptyl isothiocyanate, were significantly upregulated after RSG extract intervention. Furthermore, enrichment analysis of metabolic pathways showed that the anti-obesity effects of RSG extract may be mediated by alterations in bile secretion, fat digestion and absorption, and biosynthesis of plant secondary metabolites. Overall, RSG extract can inhibit the development of obesity, and the obesity-alleviating effects of RSG are related to alternative regulation of the gut microbiota and
glucosinolate metabolites.