背景:曲霉病的治疗选择包括两性霉素B(AMB)和唑类化合物,例如伊曲康唑(ITZ)。然而,与这些抗真菌药物相关的严重副作用越来越明显,阻力继续增加。目前,解决这一问题的药物发现的新趋势以植物的天然产物为代表,或他们的提取物。特别是,人们对精油(EO)非常感兴趣,因为它们对细菌具有抗菌作用,真菌和病毒。
方法:在本研究中,我们评估了11种商业EOs丁香的抗真菌活性,桉树,天竺葵,混合薰衣草,薰衣草,柠檬,柠檬草,neroli,牛至,茶树和红百里香-与AMB和ITZ对黄曲霉的比较,烟曲霉和黑曲霉临床分离株。通过肉汤微量稀释法测定抗真菌活性,琼脂扩散技术,抑菌和杀真菌活性和蒸汽接触测定。
结果:气相色谱-质谱分析显示两组不同的生物合成来源:单萜在大多数油的化学成分中占主导地位。丁香EO中只有两种芳香族化合物(丁香酚78.91%和乙酸丁香酯11.64%)被确定为主要成分。柠檬草EO表现出最强的抗菌活性,对曲霉属的最低抑菌浓度为0.56mg/mL,最低杀菌浓度为2.25-4.5mg/mL。菌株。丁香和天竺葵EO对抑制曲霉属相当有效。增长。
结论:这些结果证明了某些EOs的抗菌潜力,并支持基于EOs的新替代品或补充疗法的研究。
BACKGROUND: Treatment options for aspergillosis include amphotericin B (AMB) and azole compounds, such as itraconazole (ITZ). However, serious side effects related to these antifungal agents are increasingly evident, and resistance continues to increase. Currently, a new trend in drug discovery to overcome this problem is represented by natural products from plants, or their extracts. Particularly, there is a great interest in essential oils (EOs) recognized for their antimicrobial role towards bacteria, fungi and viruses.
METHODS: In this study, we evaluated the antifungal activity of eleven commercial EOs-clove, eucalyptus, geranium, hybrid lavender, lavender, lemon, lemongrass, neroli, oregano, tea tree and red red thyme-in comparison with AMB and ITZ against Aspergillus flavus, A. fumigatus and A. niger clinical isolates. Antifungal activity was determined by broth microdilution method, agar diffusion technique, fungistatic and fungicidal activities and vapor contact assay.
RESULTS: Gas chromatography-mass spectrometry analysis displayed two groups of distinct biosynthetical origin: monoterpenes dominated the chemical composition of the most oils. Only two aromatic compounds (eugenol 78.91% and eugenyl acetate 11.64%) have been identified as major components in clove EO. Lemongrass EO exhibits the strongest antimicrobial activity with a minimum inhibitory concentration of 0.56 mg/mL and a minimum fungicidal concentration of 2.25-4.5 mg/mL against Aspergillus spp. strains. Clove and geranium EOs were fairly effective in inhibiting Aspergillus spp. growth.
CONCLUSIONS: These results demonstrate the antimicrobial potential of some EOs and support the research of new alternatives or complementary therapies based on EOs.