UNASSIGNED:自2007年格鲁吉亚首次报告非洲猪瘟(ASF)爆发以来,该病已扩展到欧洲,俄罗斯,亚洲,通过与包括家猪和野猪在内的受感染动物接触而迅速传播。这种II型非洲猪瘟病毒(ASFV)在广泛的地区和宿主中的广泛扩展不可避免地导致了新突变的获得。这些突变可用于追踪ASFV的分子流行病学,前提是它们是限制在一定区域内的菌株所独有的。虽然全基因组测序仍然是检查进化变化的黄金标准,具有显著变异和分辨能力的单个基因座的测序可用作快速且具有成本效益的替代方案,以表征来自单个或相关爆发的多个分离株。
UNASSIGNED:对2017年至2019年俄罗斯加里宁格勒地区活跃的ASF爆发期间获得的ASFV进行了检查。由于所有的病毒都属于基因型II,并且没有观察到基于中央可变区(CVR)测序的明确分化,测定了该区域9个ASFV分离株的全基因组序列.为了深入了解这些分离株的分子进化,将它们的序列与来自欧洲的分离株进行比较,亚洲,和非洲。
UNASSIGNED:基于全基因组序列的系统发育分析将新分离株聚集为波兰和德国分离株的姐妹谱系。这表明可能的共同起源,然后添加了仅限于该区域分离株的新突变。在分析MGF-505-5R和MGF-110-7L的多态性时,这种姐妹谱系的状态得到了反映,同时在K145R基因座鉴定出加里宁格勒序列特有的多态性。这种新鉴定的突变能够区分从加里宁格勒获得的分离株与GenBank上可获得的基因型IIASFV的序列。
UNASSIGNED:这项研究的结果表明,在加里宁格勒循环的ASFV最近获得了这种突变,为先前描述的突变提供了额外的标记。
UNASSIGNED: Since the first report of outbreaks of African swine fever (ASF) in Georgia in 2007, the disease has expanded into Europe, Russia, and Asia, spreading rapidly via contact with infected animals including domestic pigs and wild boars. The vast expansion of this Genotype II African swine fever virus (ASFV) across wide-ranging territories and hosts inevitably led to the acquisition of novel mutations. These mutations could be used to track the molecular epidemiology of ASFV, provided that they are unique to strains restricted within a certain area. Whilst whole-genome sequencing remains the gold standard for examining evolutionary changes, sequencing of a single locus with significant variation and resolution power could be used as a rapid and cost-effective alternative to characterize multiple isolates from a single or related outbreak.
UNASSIGNED: ASFVs obtained during active ASF outbreaks in the Russian region of Kaliningrad between 2017 and 2019 were examined. Since all of the viruses belonged to Genotype II and no clear differentiation based on central variable region (CVR) sequencing was observed, the whole-genome sequences of nine ASFV isolates from this region were determined. To obtain insights into the molecular evolution of these isolates, their sequences were compared to isolates from Europe, Asia, and Africa.
UNASSIGNED: Phylogenetic analysis based on the whole-genome sequences clustered the new isolates as a sister lineage to isolates from Poland and Germany. This suggests a possible shared origin followed by the addition of novel mutations restricted to isolates from this region. This status as a sister lineage was mirrored when analyzing polymorphisms in MGF-505-5R and MGF-110-7L, whilst a polymorphism unique to sequences from Kaliningrad was identified at locus K145R. This newly identified mutation was able to distinguish the isolates obtained from Kaliningrad with sequences of Genotype II ASFVs available on GenBank.
UNASSIGNED: The findings of this study suggest that ASFVs circulating in Kaliningrad have recently obtained this mutation providing an additional marker to the mutations previously described.