背景:运动型马的运动性肺出血(EIPH)的特征是剧烈运动后气管支气管树中存在来自肺部的血液。尽管EIPH在马匹中的患病率很高,主要病因尚不清楚。编码CD39和CD39L1(分别为ENTPD1和ENTPD2)的基因中的变异体先前被报道为涉及EIPH发病机理的潜在遗传原因。然而,这些变异体在止血功能中的作用尚不清楚.
结果:为了研究EIPH与ENTPD1(rs1152296272,rs68621348和rs68621347)和ENTPD2基因(rs782872967)中错义变体之间的关联,对76匹诊断为EIPH的纯种马和56匹通过气管-支气管内窥镜检查无EIPH临床体征的纯种马(对照组)进行基因分型。rs1152296272和rs68621347变体是连接的,这解释了为什么在所有马匹中都发现了相同的结果。大约96%和95%的EIPH和对照马,分别,携带这些变体的至少一个非参考等位基因。相比之下,100%的对照马和96%的EIPH马对于rs68621348变体的参考等位基因是纯合的。在EIPH组中,对于rs782872967变体的非参考等位基因,1.5%的马是纯合子,24%是杂合的。在对照组中,仅在杂合子中观察到该变体的非参考等位基因(16%).对于任何变体,组间没有显著差异。
结论:先前在编码CD39和CD39L1酶的基因中描述的变体在研究群体中高度存在。然而,在这项研究中,在纯种马中没有发现EIPH的发生和这些变异的存在之间的关联.
BACKGROUND: Exercise-induced pulmonary haemorrhage (EIPH) in athletic horses is characterized by the presence of blood from the lungs in the tracheobronchial tree after intense exercise. Despite the high prevalence of EIPH in horses, the primary aetiology remains unknown. Variants in the genes encoding CD39 and CD39L1 (ENTPD1 and ENTPD2, respectively) were previously reported as potential genetic causes involved in EIPH pathogenesis. However, the role of these variants in haemostatic functions is unknown.
RESULTS: To investigate the association between EIPH and missense variants in the ENTPD1 (rs1152296272, rs68621348, and rs68621347) and ENTPD2 genes (rs782872967), 76 Thoroughbred horses diagnosed with EIPH and 56 without clinical signs of EIPH (control group) by trachea-bronchial endoscopy were genotyped. The rs1152296272 and rs68621347 variants were linked, which explained why the same results were found in all horses. Approximately 96% and 95% of the EIPH and control horses, respectively, carried at least one nonreference allele for these variants. In contrast, 100% of the control horses and 96% of the EIPH horses were homozygous for the reference allele for the rs68621348 variant. In the EIPH group, 1.5% of the horses were homozygotes and 24% were heterozygous for the nonreference allele of the rs782872967 variant. In the control group, the nonreference allele of this variant was observed only in heterozygotes (16%). There were no significant differences between groups for any of the variants.
CONCLUSIONS: The variants previously described in the genes encoding the CD39 and CD39L1 enzymes were highly present in the studied population. However, no association was found between the occurrence of EIPH and the presence of these variants in Thoroughbred horses in this study.