■植入前基因检测(PGT)可以降低家族性遗传病的风险,染色体异常,和反复流产。目前尚不清楚具有PGT适应症的遗传顾问是否理解并接受PGT的含义。需要一个完善和验证的工具来评估知识,态度,具有PGT适应症的遗传顾问的实践(KAP)水平。这项研究的目的是为具有PGT适应症的遗传顾问开发和验证PGTKAP问卷(PGT-KAP-Q)。
■首先,我们在文献综述和定性访谈的基础上建立了项目库。第二,我们使用德尔菲法开发了PGT-KAP-Q。第三,我们使用项目分析和心理测量学评估来评估问卷的质量.项目分析包括极值比较,相关性和Cronbachα(α)系数方法的应用,和因素分析。我们还评估了问卷的内容和结构效度,以及内部一致性,测试-重测可靠性,和半分割的可靠性。
■经过文献回顾和访谈,在三轮专家磋商的基础上,我们形成了一个43项问卷。在有效性分析中,项目的内容效度指数(I-CVI)和平均量表水平CVI(S-CVI/Ave)值(分别>0.78和>0.95)证实了问卷的内容效度。探索性因素分析表明,所有43个项目都有较强的因子负荷(>0.4),PGT-KAP-Q三个因子解释了总方差的51.97%。问卷的克朗巴赫α系数为0.95(p<0.05),分裂半信度为0.76(p<0.05),重测信度系数为0.78(p<0.05)。
■针对具有PGT适应症的遗传顾问的43项PGT-KAP-Q是可靠且有效的。它包含适度数量的项目,患者容易理解和接受,可用于临床研究和应用。
UNASSIGNED: Preimplantation genetic testing (PGT) can reduce the risk of familial genetic diseases, chromosome abnormalities, and recurrent abortions. It is unclear whether genetic counselees with PGT indications understand and accept the implications of PGT. A well-developed and validated tool is needed to evaluate the knowledge, attitude, and practice (KAP) levels of genetic counselees with PGT indications. The purpose of this study was to develop and validate a PGT KAP questionnaire (PGT-KAP-Q) for genetic counselees with PGT indications.
UNASSIGNED: First, we established an item pool based on a literature review and qualitative interviews. Second, we developed the PGT-KAP-Q using the Delphi method. Third, we evaluated the quality of the questionnaire using item analysis and psychometric evaluation. The item analysis included extreme value comparison, application of the correlation and Cronbach\'s alpha (α) coefficient methods, and factor analysis. We also evaluated the content and structural validity of the questionnaire, as well as the internal consistency, test-retest reliability, and split-half reliability.
UNASSIGNED: After the literature review and interviews, and based on three rounds of expert consultations, we formed a 43-item questionnaire. In the validity analysis, the item\'s content validity index (I-CVI) and the average scale level CVI (S-CVI/Ave) values (>0.78 and >0.95, respectively) confirmed the questionnaire\'s content validity. Exploratory factor analysis showed that all 43 items had strong factor loadings (>0.4), and the three factors of the PGT-KAP-Q explained 51.97 % of the total variance. The Cronbach\'s α coefficient for the questionnaire was 0.95 (p < 0.05), the split-half reliability was 0.76 (p < 0.05) and the test-retest reliability coefficient was 0.78 (p < 0.05).
UNASSIGNED: The 43-item PGT-KAP-Q for genetic counselees with PGT indications is reliable and valid. It contains a moderate number of items, is easy for patients to understand and accept, and can be used for clinical research and applications.