■本研究旨在调查C3基因位点的9个标签单核苷酸多态性(SNPs)与中国人群冠心病(CAD)风险和血脂水平之间的关联。并进一步探讨SNP与可能与CAD风险相关的环境因素之间的相互作用。
■进行了一项病例对照研究,以调查医院环境中CAD和C3基因多态性之间的关联。该研究包括944名平均年龄为55.97±10.182岁的CAD患者和897名平均年龄为55.94±9.162岁的非CAD对照。CAD组有565名男性和288名女性,对照组有583名男性和314名女性。通过使用改进的多重连接检测反应(iMLDR)技术鉴定C3基因中的TagSNPs,多因素降维(MDR)分析用于研究C3基因-环境和基因-基因相互作用与CAD风险的关系。
■多态性研究结果表明,与对照组相比,CAD组rs7257062的CC基因型更频繁(10.9%vs7.7%),具有统计学上的显著差异(p=0.009)。此外,CAD亚组rs7257062的TT和CC+CT基因型组的血清甘油三酯水平存在显著差异(2.326±1.889vs2.059±1.447,p=0.019).总胆固醇(TC)分析,低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C),载脂蛋白A(ApoA),载脂蛋白B(ApoB)水平显示TT和CCCT基因型之间没有显着差异。此外,其他SNP基因型之间的血脂水平没有显着差异。多变量逻辑分析,控制性别,年龄,体重指数(BMI),甘油三酯(TG),TC,HDL-C,LDL-C,ApoA和ApoB,结果证明rs7257062仍是CAD的独立危险因素(OR=1.499,95%CI:1.036~2.168,p=0.032)。MDR分析表明,rs7257062与吸烟等环境因素有显著的相互作用,糖尿病,高血压,BMI,和TG(p<0.05)。
■C3基因的rs7257062变异可能与脂质平衡和CAD的风险有关。可以想象,C3多态性与环境因素之间的相互作用可以解释CAD的病因。
UNASSIGNED: This study aims to investigate the association between nine tag single nucleotide polymorphisms (SNPs) in the C3 gene locus and the risk of coronary artery disease (CAD) as well as lipid levels in the Chinese population, and to further explore the interactions between SNPs and environmental factors that may be associated with CAD risk.
UNASSIGNED: A case-control study was conducted to investigate the association between CAD and C3 gene polymorphisms in a hospital setting. The study consisted of 944 CAD patients with a mean age of 55.97 ± 10.182 years and 897 non-CAD controls with a mean age of 55.94 ± 9.162 years. There were 565 males and 288 females in the CAD group and 583 males and 314 females in the control group. TagSNPs in the C3 gene were identified by employing the improved multiplex ligation detection reaction (iMLDR) technique, and multifactor dimensionality reduction (MDR) analysis was utilized to investigate the C3 gene-environment and gene-gene interactions in relation to the risk of CAD.
UNASSIGNED: Results of the polymorphism study indicated that the CC genotype of rs7257062 was more frequent in the CAD group compared to the control group (10.9% vs 7.7%), with a statistically significant difference (p = 0.009). Moreover, the TT and CC + CT genotype groups of rs7257062 in the CAD subgroup showed a significant difference in terms of serum triglyceride levels (2.326 ± 1.889 vs 2.059 ± 1.447, p = 0.019). Analysis of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), apolipoprotein A (ApoA), and apolipoprotein B (ApoB) levels revealed no significant differences between the TT and CC + CT genotypes. Furthermore, no significant differences in serum lipid levels were observed between genotypes of the other SNPs. Multivariable logistic analysis, controlling for gender, age, body mass index (BMI), triglycerides (TG), TC, HDL-C, LDL-C, ApoA and ApoB, demonstrated that rs7257062 was still an independent risk factor of CAD (OR = 1.499, 95% CI: 1.036-2.168, p = 0.032). MDR analysis revealed that the rs7257062 interacted significantly with environmental factors such as smoking, diabetes, hypertension, BMI, and TG (p < 0.05).
UNASSIGNED: The rs7257062 variation of the C3 gene could be linked to both lipid balance and the risk of CAD. It is conceivable that the interplay between C3 polymorphisms and environmental elements could account for the etiology of CAD.