■该研究旨在通过全面评估病例对照研究,系统地确定在胃癌中观察到的肠道微生物群的变化。
■PubMed的系统文献检索,Embase,科克伦图书馆,和WebofScience进行了病例对照研究,这些研究比较了患有和不患有胃癌的个体的微生物群。采用纽卡斯尔-渥太华质量评价量表(NOS)对纳入研究的质量进行评价。荟萃分析利用随机效应模型,进行亚组和敏感性分析以评估研究异质性.所有数据分析均使用Stata17.0中的“metan”软件包进行,并使用具有95%置信区间(CI)的对数比值比(logOR)描述结果。
■共有33项研究涉及4,829名参与者有资格进行分析,其中29项研究提供了α多样性的变化,18项研究报告了β多样性。Meta分析显示,只有Shannon指数对α-多样性有统计学意义[-5.078(-9.470,-0.686)]。在门水平上没有观察到显著差异,虽然11种细菌在属水平上被鉴定出显著变化,例如,乳杆菌[5.474,(0.949,9.999)]和链球菌[5.095,(0.293,9.897)]的增加,而卟啉单胞菌和Rothia的减少则相同[-8.602,(-11.396,-5.808)]。敏感性分析表明,9个细菌属的变化是稳健的。对国家的亚组分析显示,患有胃癌的韩国人中,螺杆菌和链球菌的丰度越来越高,而来自葡萄牙的胃癌患者的奈瑟氏球菌减少。关于样本来源,该研究观察到胃癌患者胃粘膜中乳杆菌和拟杆菌的增加,以及螺杆菌和链球菌。然而,与非胃癌组相比,拟杆菌的相对丰度降低,在粪便样本中显示。
■这项研究确定了胃癌患者中9种细菌属的强烈变化,这是特定于国家/样本来源的。需要进行大规模的研究来探索这些变化背后的机制。
■唯一标识符:CRD42023437426https://www。crd.约克。AC.uk/prospro/display_record.php?ID=CRD42023437426。
UNASSIGNED: The study aims to systematically identify the alterations in gut microbiota that observed in gastric cancer through comprehensive assessment of case-control studies.
UNASSIGNED: The systematic literature search of PubMed, Embase, Cochrane Library, and Web of Science was conducted to identify case-control studies that compared the microbiomes of individuals with and without gastric cancer. Quality of included studies was evaluated with the Newcastle-Ottawa Quality Assessment Scale (NOS). Meta-analyses utilized a random-effects model, and subgroup and sensitivity analyses were performed to assess study heterogeneity. All data analyses were performed using the \"metan\" package in Stata 17.0, and the results were described using log odds ratios (log ORs) with 95% confidence intervals (CIs).
UNASSIGNED: A total of 33 studies involving 4,829 participants were eligible for analysis with 29 studies provided changes in α diversity and 18 studies reported β diversity. Meta-analysis showed that only the Shannon index demonstrated statistical significance for α-diversity [-5.078 (-9.470, -0.686)]. No significant differences were observed at the phylum level, while 11 bacteria at genus-level were identified significant changed, e.g., increasing in Lactobacillus [5.474, (0.949, 9.999)] and Streptococcus [5.095, (0.293, 9.897)] and decreasing in Porphyromonas and Rothia with the same [-8.602, (-11.396, -5.808)]. Sensitivity analysis indicated that the changes of 9 bacterial genus were robust. Subgroup analyses on countries revealed an increasing abundance of Helicobacter and Streptococcus in Koreans with gastric cancer, whereas those with gastric cancer from Portugal had a reduced Neisseria. Regarding the sample sources, the study observed an increase in Lactobacillus and Bacteroides in the gastric mucosa of people with gastric cancer, alongside Helicobacter and Streptococcus. However, the relative abundance of Bacteroides decreased compared to the non-gastric cancer group, which was indicated in fecal samples.
UNASSIGNED: This study identified robust changes of 9 bacterial genus in people with gastric cancer, which were country-/sample source-specific. Large-scale studies are needed to explore the mechanisms underlying these changes.
UNASSIGNED: Unique Identifier: CRD42023437426 https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023437426.