PDF(Pubmed)
Abstract：
H. pylori infection is gaining increasing attention, but detailed investigations into its impact on gastric microbiota remain limited. We collected gastric mucosa samples from 47 individuals divided into three groups: 1. Group HP: patients with initial positive H. pylori infection (25 cases); 2. Group ck: H. pylori-negative patients (14 cases); 3. Group DiffHP: patients with refractory H. pylori infection (8 cases). The samples were analyzed using 16S rDNA sequencing and functional prediction with PICRUSt. Group HP showed differences in flora distribution and function compared to Group ck, while Group DiffHP overlapped with Group HP. The abundances of Aeromonas piscicola, Shewanella algae, Vibrio plantisponsor, Aeromonas caviae, Serratia marcescens, Vibrio parahaemolyticus, Microbacterium lacticum, and Prevotella nigrescens were significantly reduced in both Group DiffHP and Group HP compared to Group ck. Vibrio shilonii was reduced only in Group DiffHP compared to Group ck, while Clostridium perfringens and Paracoccus marinus were increased only in Group DiffHP. LEfSe analysis revealed that Clostridium perfringens and Paracoccus marinus were enriched, whereas Vibrio shilonii was reduced in Group DiffHP compared to Group ck at the species level. In individuals with refractory H. pylori infection, the gastric microbiota exhibited enrichment in various human diseases, organic systems, and metabolic pathways (amino acid metabolism, carbohydrate metabolism, transcription, replication and repair, cell cycle pathways, and apoptosis). Patients with multiple failed H. pylori eradication exhibited significant changes in the gastric microbiota. An increase in Clostridium perfringens and Paracoccus marinus and a decrease in Vibrio shilonii appears to be characteristic of refractory H. pylori infection.
摘要：
幽门螺杆菌感染越来越受到关注，但对其对胃微生物群影响的详细研究仍然有限.我们收集了47名个体的胃粘膜样本，分为三组：1.HP组：H.pylori感染初始阳性患者（25例）；2.ck组：H.pylori阴性患者（14例）；3.DiffHP组：难治性幽门螺杆菌感染患者（8例）。使用16SrDNA测序和PICRUSt功能预测对样品进行分析。HP组显示与ck组相比，在菌群分布和功能上存在差异。而DiffHP组与HP组重叠。piscicola气单胞菌的丰度，Shewanella藻类，弧菌赞助，鱼气单胞菌,粘质沙雷菌,副溶血性弧菌,乳杆菌,与ck组相比，DiffHP组和HP组的黑质Prevotella均显着减少。与ck组相比，Shilonii弧菌仅在DiffHP组中减少，而产气荚膜梭状芽胞杆菌和paracocus仅在DiffHP组中增加。LEfSe分析显示产气荚膜梭菌和paracocusparinus富集，而在物种水平上，DiffHP组的shilonii弧菌比ck组减少。在患有难治性幽门螺杆菌感染的个体中，胃微生物在各种人类疾病中表现出富集，有机系统，和代谢途径(氨基酸代谢，碳水化合物代谢，转录,复制和修复，细胞周期通路，和凋亡）。多次根除幽门螺杆菌失败的患者在胃微生物群中表现出显著的变化。产气荚膜梭状芽胞杆菌和paracoccusmarinus的增加和shilonii弧菌的减少似乎是难治性幽门螺杆菌感染的特征。
