■这项研究旨在评估妊娠后期和哺乳期饮食中低聚半乳糖(GOS)和猪胆酸(HCA)对生殖性能的影响,初乳质量,多产母猪的抗氧化能力和肠道菌群。
■共60头健康多胎杂交母猪(长白猪×约克郡)随机饲喂4组饮食:基础饮食(CTRL组),或仅含600mg/kgGOS的基础饮食(GOS组),600mg/kgGOS+100mg/kgHCA(GOS+低HCA组),从妊娠到断奶的第85天,600mg/kg+200mg/kgHCA(GOS+高HCA组)。确定了母猪的多个参数。
■在GOS组和GOS低/高HCA组中存在缩短母猪产程的趋势(p=0.07)。与CTRL组相比,GOS+低/高HCA组增加了仔猪出生时的平均体重(p<0.05),并增加初乳的IgA浓度(p<0.05)。此外,血清甘油三酯(TG)浓度较低(p<0.05),分娩时,GOS和GOS低/高HCA组的血清总抗氧化能力(T-AOC)高于CTRL组(p<0.05)。分娩时,GOS和GOS高HCA组的血清过氧化氢酶(CAT)活性高于CTRL组。16SrRNA分析表明,GOS与高剂量HCA的组合塑造了不同生殖阶段的肠道微生物群的组成(妊娠的d107,G107;泌乳d0,L0;泌乳d7,L7).在门一级,G107中的类细菌和脱硫细菌的相对丰度,类细菌,在GOS高HCA组中,L0和L7的Planctomycetota增加(p<0.05)。Spearman相关分析表明,链球菌与血清TG呈正相关,与仔猪出生时平均体重呈负相关(p<0.05)。
■这项研究表明,低聚半乳糖(GOS)与猪胆酸(HCA)联合使用,妊娠后期和哺乳期营养受限的母猪,进一步提高了它们的抗氧化能力和牛奶质量。观察到的GOS+HCA补充的有益效果可能与母猪的肠道微生物群紊乱的改善有关。
UNASSIGNED: This study was conducted to evaluate the effects of dietary galacto-oligosaccharides (GOS) and hyocholic acids (HCA) during late gestation and lactation on reproductive performance, colostrum quality, antioxidant capacity and gut microbiota in multiparous sows.
UNASSIGNED: A total of 60 healthy multiparous cross-bred sows (Landrace × Yorkshire) were randomly fed 4 groups diets as follows: the basal diets (CTRL group), or the basal diets containing only 600 mg/kg GOS (GOS group), 600 mg/kg GOS + 100 mg/kg HCA (GOS + Low HCA group), and 600 mg/kg + 200 mg/kg HCA (GOS + High HCA group) from d 85 of gestation to weaning. Multiple parameters of sows were determined.
UNASSIGNED: There was a trend of shortening the labor process of sows (p = 0.07) in the GOS group and GOS + Low/High HCA group. Compared with the CTRL group, the GOS + Low/High HCA group increased the average piglets weight at birth (p < 0.05), and increased the IgA concentration of colostrum (p < 0.05). In addition, serum triglyceride (TG) concentration was lower (p < 0.05), and serum total antioxidant capacity (T-AOC) was higher (p < 0.05) in the GOS and GOS + Low/High HCA groups than in the CTRL group at farrowing. Serum catalase (CAT) activities was higher in the GOS and GOS + High HCA groups than in the CTRL group at farrowing. The 16S rRNA analysis showed that GOS combination with high-dose HCA shaped the composition of gut microbiota in different reproductive stages (d 107 of gestation, G107; d 0 of lactation, L0; d 7 of lactation, L7). At the phylum level, the relative abundance of Bacteroidota and Desulfobacterota in G107, Bacteroidota, and Proteobacteria in L0, and Planctomycetota in L7 was increased in GOS + High HCA group (p < 0.05). Spearman correlation analysis showed that Streptococcus was positively correlated with the serum TG but negatively correlated with the average piglets weight at birth (p < 0.05).
UNASSIGNED: This investigation demonstrated that the administration of galacto-oligosaccharides (GOS) in conjunction with hyocholic acids (HCA), to sows with nutrient restrictions during late gestation and lactation, further improved their antioxidant capacity and milk quality. The observed beneficial effects of GOS + HCA supplementation could potentially be linked to an improvement in gut microbiota disorders of the sows.