灰色模具,由葡萄孢菌引起。,是哥伦比亚玫瑰作物中的一种重要疾病,其控制主要取决于化学合成的杀真菌剂的大量使用。尽管这种病原体很重要,哥伦比亚花卉栽培中关于物种分子分类学的信息有限,种群对杀菌剂的抗性及其抗性的遗传机制。在这项研究中,我们分析了从昆迪纳马卡系的玫瑰生产作物中收集的这种真菌的12种分离物,并使用HSP60,G3PDH进行了系统发育分析,和RPB2基因序列。此外,我们意识到对杀菌剂fenhexamid抗性的表型和基因型表征,羧基,和丙草胺,评估每个分离株的体外EC50和靶基因突变的存在。在系统发育分析中,所有分离株都被表征为灰葡萄孢菌,并对每种杀菌剂表现出不同水平的抗性。这些水平与靶基因的突变有关,在ERG27基因中以L195F和L400F为主,H272R/Y在SDHB基因中对羧基蛋白抗性,和CYP51基因中的Y136F对丙草胺抗性。最后,这些突变与形态学改变无关.总的来说,这些知识,首次提交给哥伦比亚花卉种植,有助于更好地了解昆迪纳马卡省玫瑰生产作物的B.cinerea的遗传多样性和种群,并作为做出有关疾病管理的明智决策的宝贵工具,未来的研究,并改善哥伦比亚花卉产业的作物管理和可持续性。
Gray mold, caused by Botrytis sp., is a significant disease in Colombian rose crops and its control depends primarily on the intensive use of chemically synthesized fungicides. Despite the importance of this pathogen, there is limited information in Colombian floriculture about molecular taxonomy of species,
fungicide resistance of populations and their genetic mechanism of resistance. In this study, we analyze 12 isolates of this fungus collected from rose-producing crops in the Department of Cundinamarca and conducted phylogenetic analysis using HSP60, G3PDH, and RPB2 gene sequences. Additionally, we realize phenotypic and genotypic characterization of resistance to the fungicides fenhexamid, carboxin, and prochloraz, evaluating the in vitro EC50 and presence of mutations of target genes of each isolate. All isolates were characterized as Botrytis cinerea in the phylogenetic analysis and presents different levels of resistance to each
fungicide. These levels are related to mutations in target genes, with predominancy of L195F and L400F in the ERG27 gene to fenhexamid resistance, H272R/Y in the SDHB gene for carboxin resistance, and Y136F in the CYP51 gene for prochloraz resistance. Finally, these mutations were not related to morphological changes. Collectively, this knowledge, presented for the first time to the Colombian floriculture, contribute to a better understanding of the genetic diversity and population of B. cinerea from rose-producing crops in the department of Cundinamarca, and serve as a valuable tool for making informed decisions regarding disease management, future research, and improving crop management and sustainability in the Colombian floriculture industry.