放线植物的根瘤是固氮细菌共生体的家园,被称为Frankia,以及一小部分其他微生物。这些包括内生真菌和非Frankia细菌。这些根瘤中微生物聚生体的分类学和功能多样性尚不清楚。在这项研究中,我们调查和分析了可培养的,来自红色和Sitkaal树的根瘤的非Frankia真菌和细菌内生菌。我们检查了它们的分类多样性,共现,主机之间的差异,和潜在的职能角色。第一次,我们报告了大量的醛固酮根瘤内生真菌。这些包括齿形孢子丝菌,Fontanosporasp.,Cadobhoramelinii,一个未分类的Cadobhora,Ilyonectria破坏,一个未分类的Gibberella,ramulariae,一种未分类的木霉,塔西亚菌丝菌,未分类的Talaromyces,Coniochaetasp.,还有Sistotremabrinkmanii.我们还首次报道了几个细菌属:Collimonas,嗜冷杆菌,和Phylbacterium。此外,我们第二次报道沙雷氏菌属,第一份报告最近于2023年发布。假单胞菌是最常分离的细菌属,被发现与真菌和细菌共同栖息在单个结节中。我们发现真菌内生菌的群落因宿主物种而异,而内生细菌的群落却没有。
The root nodules of actinorhizal plants are home to nitrogen-fixing bacterial symbionts, known as Frankia, along with a small percentage of other microorganisms. These include fungal endophytes and non-Frankia bacteria. The taxonomic and functional diversity of the microbial consortia within these root nodules is not well understood. In this study, we surveyed and analyzed the cultivable, non-Frankia fungal and bacterial endophytes of root nodules from red and Sitka alder trees that grow together. We examined their taxonomic diversity, co-occurrence, differences between hosts, and potential functional roles. For the first time, we are reporting numerous fungal endophytes of alder root nodules. These include Sporothrix guttuliformis, Fontanospora sp., Cadophora melinii, an unclassified Cadophora, Ilyonectria destructans, an unclassified Gibberella, Nectria ramulariae, an unclassified Trichoderma, Mycosphaerella tassiana, an unclassified Talaromyces, Coniochaeta sp., and Sistotrema brinkmanii. We are also reporting several bacterial genera for the first time: Collimonas, Psychrobacillus, and Phyllobacterium. Additionally, we are reporting the genus Serratia for the second time, with the first report having been recently published in 2023. Pseudomonas was the most frequently isolated bacterial genus and was found to co-inhabit individual nodules with both fungi and bacteria. We found that the communities of fungal endophytes differed by host species, while the communities of bacterial endophytes did not.