PDF(Pubmed)
Abstract：
Non-insecticidal control strategies using entomopathogens, nematodes, and endophytes provide sustainable and safer alternatives for managing crop pests. This study investigated the potential of different fungal endophytes, specifically Beauveria bassiana strains, in colonizing cotton plants and their efficacy against tarnished plant bug, Lygus lineolaris. The effect of endophytes on plant growth parameters and cotton yield were measured during different plant growth stages. The entomopathogenicity of these fungi was studied in diet cup bioassays using L. lineolaris adults. The behavior of adult males and females toward endophytic cotton squares was analyzed using olfactometer assays. The experiments showed that the fungal endophytes colonized the plant structures of cotton plants, which resulted in an increase in the number of cotton squares, plant height, and weight compared to control plants. B. bassiana strains/isolates such as GHA, NI-8, and JG-1 caused significant mortality in Lygus adults compared to controls. Also, male and female Lygus adults exhibited repellence behavior towards endophytic cotton squares containing JG-1 isolate of B. bassiana and to other B. bassiana strains such as NI-8, GHA, and SPE-120. No differences were observed in the survival and development of L. lineolaris second-instar nymphs on endophytic cotton, and no yield differences were observed in the field experiments.
摘要：
使用昆虫病原体的非杀虫控制策略，线虫,和内生菌为管理作物害虫提供了可持续和更安全的替代品。这项研究调查了不同真菌内生菌的潜力，特别是白僵菌菌株，在定殖棉花植物及其对抗受损植物虫子的功效中，Lyguslineolaris.在不同的植物生长阶段，测量了内生菌对植物生长参数和棉花产量的影响。这些真菌的昆虫致病性是在饮食杯生物测定中使用成年乳杆菌研究的。使用嗅觉计测定法分析了成年雄性和雌性对内生棉花正方形的行为。实验表明，内生真菌定植于棉花植物的植物结构中，这导致棉花方块的数量增加，植物高度,与对照植物相比的重量。B.Bassiana菌株/分离株，如GHA，与对照组相比，NI-8和JG-1在成年Lygus中引起了显著的死亡率。此外，雄性和雌性Lygus成虫对含有JG-1分离株的内生棉方块表现出排斥行为。白杨和其他白杨菌株，如NI-8，GHA，SPE-120在内生棉花上，品系二龄若虫的存活和发育没有观察到差异，在田间试验中没有观察到产量差异。
