嗅觉基因家族包括气味结合蛋白(OBP),化学感觉蛋白(CSP),嗅觉受体(ORs),离子型受体(IR)和味觉受体(GR)。探讨罗氏沼虾嗅觉感知的分子功能,我们整合了全长转录本和全基因组序列以鉴定嗅觉基因家族.在这项研究中,通过PacBioSMRT测序共获得38,955个全长转录本,N50长度为3383bp。通过全长转录本和全基因组序列的注释,确定了几个嗅觉基因家族,包括18个MrORs,16MrIR,151MrIGluRs(离子型谷氨酸受体),2个MrVIGluR(变体离子型谷氨酸受体)和3个MrCRs(化学感应受体)。值得注意的是,CRs最初是在对虾和虾中发现的。此外,在日本M.nipponense中的嗅觉基因家族被鉴定出来,包括4个MnOR,21MnIR,79MnIGluR,5MnVIGluR,1MnGR和1MnOBP,使用可用的全基因组序列。同时,研究了罗森贝氏杆菌化学感觉器官的外部形态,和羽状刚毛(PS)的存在,硬刺刚毛(HTS),竹笋刚毛(BSS),触角上的软刺刚毛(STS)和美学(AE),第二个天线上的HTS和BSS,通过扫描电子显微镜观察了脚足类的PS。本研究为今后甲壳类动物嗅觉感知功能研究提供了有价值的见解,并为罗森博吉的分子设计育种奠定了理论基础。
The olfactory gene families include odorant binding proteins (OBPs), chemosensory proteins (CSPs), olfactory receptors (ORs), ionotropic receptors (IRs) and gustatory receptors (GRs). To investigate the molecular function of olfactory perception in Macrobrachium rosenbergii, we integrated the full-length transcripts and whole-genome sequences to identify the olfactory gene families. In this study, a total of 38,955 full-length transcripts with an N50 length of 3383 bp were obtained through PacBio SMRT sequencing. Through the annotation of full-length transcripts and whole-genome sequences, several olfactory gene families were identified, including 18 MrORs, 16 MrIRs, 151 MrIGluRs (ionotropic glutamate receptors), 2 MrVIGluRs (variant ionotropic glutamate receptors) and 3 MrCRs (chemosensory receptors). Notably, the CRs were first identified in prawns and shrimps. Additionally, the olfactory gene families in M. nipponense were identified, comprising 4 MnORs, 21 MnIRs, 79 MnIGluRs, 5 MnVIGluRs, 1 MnGR and 1 MnOBP, using the available whole-genome sequences. Meanwhile, the external morphology of the chemical sensory organs of M. rosenbergii was explored, and the presence of plumose setae (PS), hard thorn setae (HTS), bamboo shoot setae (BSS), soft thorn setae (STS) and aesthetascs (AE) on the antennules, HTS and BSS on the second antennae, and PS on the pereiopods were observed by scanning electron microscope. This study provides valuable insights for future functional studies into the olfactory perception of crustaceans and establishes a theoretical basis for molecular design breeding in M. rosenbergii.