Pollution of the natural environment by microplastics has become a global issue in ecosystems as it poses a potential long-term threat to biota. Microplastics can accrue in high abundances in sediments of aquatic ecosystems while also contaminating pelagic filter feeders, which could transfer pollutants up trophic webs. We assess the abundance and distribution of microplastics in benthic sediments and Cladocera taxa in a subtropical Austral reservoir using a combination of geospatial techniques, physicochemical analyses, diversity indices, and multivariate statistics between two seasons (i.e., hot-wet and cool-dry). We found particularly high densities of microplastics during the cool-dry season for both sediments (mean 224.1 vs. 189 particles kg-1 dry weight) and Cladocera taxa (0.3 particles per individual). Cladocera microplastic shapes were dominated by fibers with high densities of the transparent color scheme. Pearson correlation results indicated that sediment microplastic abundances were negatively correlated with chlorophyll-a concentration, temperature, and resistivity, whereas they were positively correlated with pH and salinity during the hot-wet season, with no variables significant in the cool-dry season. Cladocera microplastic abundances were positively correlated with conductivity and salinity during the cool-dry season, but no variables in the hot-wet season. These findings provide insights into the role of reservoirs as microplastic retention sites and the potential for uptake and transfer from lower trophic groups. These insights can be used to strengthen future monitoring and intervention strategies. Integr Environ Assess Manag 2024;1-15. © 2024 SETAC.

Microplastic has emerged as a global threat owing to its chronic ubiquity and persistence. Microplastics\' small size expedites their ingestion at each trophic level causing biomagnification and bioaccumulation, which has raised public concerns. The present study isolated, quantified and characterized the abundance, shape, size, color, and chemical composition of the microplastics from water and sediments of the Hirakud Reservoir through a scanning electron microscope and FTIR. The ecological risk associated with the microplastics was assessed using the species sensitivity distribution (SSD) method to derive the Predicted No-Effect Concentration (PNEC) value and risk quotient (RQ). The abundance of microplastics in the surface water and sediments of the Hirakud Reservoir was estimated at 82-89 particles/L and 159-163 particles/kg, respectively. Fiber-shaped microplastics dominated both surface water (46.21%) and sediment samples (44.86%). Small-sized microplastics (53-300 μm) prevailed in all samples. Color delineation exhibited an abundance of transparent microplastics. Chemical characterization indicated the dominance of polypropylene (38%), followed by high-density polyethylene, low-density polyethylene, and polystyrene. The calculated PNEC value was 3,954 particles/m3, and the RQ was estimated to be 0.02073-0.04122 indicating negligible ecological risk to freshwater species in all the sampling sites.

Microplastics and heavy metals pollution is recognised as a major problem affecting aquatic ecosystems. For this reason, this study aims to assess the toxicity of different concentrations of polyethylene microplastics (PE-MPs) (0.0, 500, and 1000 μg L-1) with a mean size of 15-25 μm and lead acetate Pb(C2H3O2)2 (0.0, 2.5, and 5 mg L-1), both individually and in combination, through the exposure of the freshwater grass shrimp, Caridinia fossarum for 15 days, focusing on microplastic interaction with co-occurring contaminants. After being exposed to both contaminants, either individually or in combination, significant alterations in numerous biochemical markers were observed. Specifically, exposure to lead acetate alone resulted in significant changes across ALP, AST, ALT, LDH, GGT, and BChE enzyme activity levels indicating hepatotoxicity and neurotoxicity. Also, Pb exposure led to alterations in total antioxidant capacity, MDA, total lipids, and glycogen contents, signalling the onset of oxidative stress. Exposure to PE-MPs alone led to changes in ALP, LDH, GGT, and BChE enzyme levels, and in MDA, total lipids, and glycogen samples\' contents. Remarkably, the study observed increased bioaccumulation of lead acetate in samples treated with the combination, emphasizing the synergistic impact of PE-MPs on the toxicity of lead acetate. This synergy was also evident in AST and ALT enzyme activity levels and MDA contents. This underscores the necessity for measures to address both microplastic pollution and heavy metal contamination, taking into account the synergistic behaviour of MPs in the presence of concurrent contaminants.

Catchment-scale understanding of water and contaminant fluxes through all pathways is essential to address land use and climate change impacts on freshwater. However, few options exist to obtain this understanding for the many catchments worldwide for which streamflow and low-frequency water chemistry, but little other data exists. We applied the Bayesian chemistry-assisted hydrograph separation and load partitioning model (BACH) to 47 catchments with widely differing characteristics. As BACH relies on concentration differences between pathways, chemodynamic behaviour of a water constituent indicates its likely suitability as tracer. Typical tracers (e.g. silica, chloride) were unavailable, but Electrical Conductivity and a few monitored nutrients proved chemodynamic in most catchments. Using one of two tracer combinations (Total Nitrogen + Electrical Conductivity, Total Nitrogen + Total Phosphorus) allowed in 85 % of the catchments to estimate streamflow contributions by near-surface (NS), shallow groundwater (SGW), and deep groundwater (DGW) pathways and pathway-specific tracer concentrations and yields with acceptable confidence. In 46 catchments, at least two pathways contributed ≥20 % of the streamflow, and all three ≥20 % in 12 catchments, cautioning against the notion of a single \'dominant\' pathway. In contrast to hydrometric hydrograph separation, BACH allows differentiation between \'young\' (NS + SGW) and \'old\' (DGW) water, which is crucial for the understanding of pollution in catchments with strong temporal gradients in land use intensity. Consistent with generally increasing land use intensity, and groundwater denitrification occurring in some catchments, Total Nitrogen (TN) concentrations were in most catchments higher in NS and SGW compared to DGW. In most catchments, the greatest fraction of the TN yield was conveyed by SGW (≈ 40-90 %). Exceptions were wet and hilly catchments under bush, where the NS transferred most of the very low yields, and three young volcanic catchments where the DGW transferred the majority of the yield due to particularly high DGW flow contributions.

While the inclusion of synthetic polymers such as primary microplastics within personal care products have been widely restricted under EU/UK Law, water-soluble polymers (WSPs) have so far slipped the net of global chemical regulation despite evidence that these could be polluting wastewater effluents at concentrations greatly exceeding those of microplastics. Polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP) represent WSPs with common industry and household uses, down-the-drain disposal and a direct route to wastewater treatment plants, conveying high risk of environmental leaching into freshwater ecosystems. The current study is the first investigating the impacts of predicted environmental concentrations of these WSPs on life-history traits of two freshwater species also constituting a disease model (fish - Poecilia reticulata and parasite - Gyrodactylus turnbulli). Single effects of WSPs on fish as well as their interactive effects with infection of the ectoparasite were determined over a 45-day exposure. Generally, WSPs reduced fish growth and increased routine metabolic rate of fish implying a depleted energetic budget, however these effects were dose, exposure time and polymer dependent. Parasitic infection alone caused a significant reduction in fish growth and enhanced fish routine metabolic rate. In contrast, a non-additive effect on metabolic rate was evident in fish experiencing simultaneous infection and WSP exposure, suggesting a protective effect of the two WSPs for fish also exposed to a metazoan ectoparasite. Off-host parasite survival was significantly lowered by both WSPs; however, parasite counts of infected fish also exposed to WSP were not significantly different from the control, implying more complex mechanisms may underpin this stressor interaction. Distinct detrimental impacts were inflicted on both organisms implying environmental leaching of WSPs may be causing significant disruption to interspecies interactions within freshwater ecosystems. Additionally, these results could contribute to sustainable development in industry, as we conclude PVA represents a less harmful alternative to PVP.

Pollution by metals is a matter of concern around the world. In recent decades, the high population growth in urban centers has significantly magnified the entry of these pollutants into aquatic ecosystems. The Amazon region, intense migratory flow, gold mining, and industrialization have been considered the main driving forces for increasing metal pollution. Thus, the main aim of this study is to conduct, for the first time, an Ecological Risk Assessment (ERA) based on metal concentrations measured in the sediment and water of several aquatic environments from the Amazon basin, based on the risk quotient values (RQ = measured environmental concentration - MEC/predicted no effect concentration - PNEC). In addition, the metal contamination factor (CF) was estimated. Although metal concentrations in water were generally low, these values were far above the limits established by current national legislation in many areas, showing higher concentrations for the metals Co, Pb, Cr, Cu, and Ni. Concentrations of Mn, Cu, Ba, Pb, Co, Ni, Cr, Zn, Cd, and As were especially high in the sediment for several evaluated environments. The ERA for the water compartment revealed that 56% of the studied areas presented high risk (RQ > 1) for aquatic biota. In the sediment, 66% of the sites presented a high risk and 40% medium risk (RQ = 0.1-1). The CF indicated that 49% of the sampling points had high contamination and only 24%, had low contamination. These results reveal that monitoring studies in the Amazon region, provides important information so that public policies for the preservation of water resources can be strengthened in the Amazon.

For nearly two years, a persistent foam cover has been observed during the post-monsoon season in the Yamuna River beneath the barrage near Okhla in Delhi, India. This affair has been a matter of public concern now, after the gigantic appearance of foam in November 2021, as the visibility of foam has awakened people\'s environmental \'conscience\' over the \'concealed\' chemical pollution. The mechanisms of agents responsible for foaming in rivers, particularly surfactants and phosphates, have received wide attention in the dynamic community of river pollution. Many studies in the past, around the globe, have evidently provided different rationales behind the dense foam formation in rivers, yet the Concerned Govt. Authorities have highlighted the cause of foam formation in the river Yamuna is associated with the presence of detergents and phosphates as foaming agents. Despite this, an aperture with copious unaccounted factors or underlying agents still exists to rationalize the foam formation and persistence. In this article, we outline these unaccounted factors which might be responsible for the foam formation and stabilization and give indications for future research directives towards the emergence of studies regarding the dense foam formation in river Yamuna.

Although the toxicity of microplastics (MPs) and pesticides has recently been described, the possible effects of combining these pollutants are poorly understood. Thus, we evaluated the potential impact of exposure to polyethylene MP (PE-MP) and abamectin (ABM) (alone and combined) in zebrafish. After five days, the combined exposure to MP and ABM decreased the survival rate compared to exposures to individual pollutants. A significant increase in reactive oxygen species (ROS), lipid peroxidation, apoptosis, and impairment in antioxidant response was observed in zebrafish larvae. Morphological changes in the eyes of zebrafish significantly increased in the combined exposure group than in the individual exposure. Furthermore, the bax and p53 expression (specific apoptotic genes) was significantly upregulated after the combined exposure to PE-MP and ABM. So, the synergetic effect of MP and ABM cannot be ignored, and further research on other higher models is required to confirm its consequences.

The main aimed of this study was to provide information on microplastics present in the freshwater of fish farm ponds. In addition, the study showes a relationship between the seasonal, spatial distribution and the amount of microplastics found. This study was conducted in 35 fish farms located in the Rondônia state, Brazil, the sample collects were carried out in the two Amazonian hydrological seasons (dry and rainy). The study was developed in a completely randomized factorial scheme 35 × 3 x 3 (35 fish farms, 3 ponds and 3 repetitions per ponds). Microplastic sampling was performed following a modified method based on National Oceanic and Atmospheric Administration (NOAA). Samples of 250 mL freshwater collected, which were deionized and pre-filtered through 6.0 mm mesh granulometric sieves. The average abundances of the different hydrological seasons were compared by Student\'s t-test, with differences statistically significant at p < 0.05. The microplastics were morphological categorized into fibers and colors blue, red or transparent. Microplastic contamination was confirmed in freshwater of 9 fish farming, with greater abundance of blue fibers and greater quantification in the rainy season. Fish farms P3, P4 and P6 had the highest quantifications of blue fiber in the two seasons (6 and 43, 19 and 56, 11 and 88 items mL-1, respectively). Almost all fish farms had a higher abundance of microplastics in the rainy season. It is important to highlight the prominence of microplastics in the blue fiber rainy season (286 items mL-1) compared to the dry season (58 items mL-1). Fish farms P3, P4 and P6 showed a strong positive correlation between the factors distance from the nearest urban area (r = 0.94, 0.79 and 0.97, respectively) and seasonality (r = 0.98, 0.77 and 0.96, respectively). Rainfall variations influenced the abundance of microplastics, especially of blue fibers. Fish farms are supplied with fresh water by rivers or streams, so it is possible that microplastics originate outside the fish farm, perhaps they were introduced due to high soil occupation, although surface runoff (of water contaminated by sewage) caused by heavy rains the most important factor. Therefore, one factor must be considered, surface runoff and groundwater contaminated by urban, agricultural and urban effluents may have contaminated rivers and streams and then contaminated the water in the fish farm ponds.

The coexistence of heavy metals (HMs) and petroleum hydrocarbons (PHs) exacerbates ecotoxicity and impair the drivers of eco-functionalities that stimulate essential nutrients for the productivity of the impacted environment. Profiling the bacteria that stem the ecological impact via HMs sequestration and PHs catabolism with nitrogen fixation is imperative to bioremediation of the polluted sites. The sediment of site that was consistently contaminated with industrial wastewaters was analysed for ecological toxicants and the bacterial strains that combined HMs resistance with PHs catabolism in a nitrogen-limiting system were isolated from the sediment and characterized. The geochemistry of the samples revealed the co-occurrence of the above-benchmark concentrations of HMs with the derivatives of hydrocarbons. Notwithstanding, nickel and mercury (with 5% each of the total metal concentrations in the polluted site) exhibited probable effect concentrations on the biota and thus hazardous to the ecosystem. Approx. 31% of the bacterial community, comprising unclassified Planococcaceae, unclassified Bradyrhizobiaceae, Rhodococcus, and Bacillus species, resisted 160 µmol Hg2+ in the nitrogen-limiting system within 24 h post-inoculation. The bacterial strains adopt volatilization, and sometimes in combination with adsorption/bioaccumulation strategies to sequester Hg2+ toxicity while utilizing PHs as sources of carbon and energy. Efficient metabolism of petroleum biomarkers (> 87%) and Hg2+ sequestration (≥ 75% of 40 µmol Hg2+) displayed by the selected bacterial strains portend the potential applicability of the bacilli for biotechnological restoration of the polluted site.