■几种荧光蛋白(FP)和色蛋白(CPs)存在于蒽虫中,并在光保护中起可能的作用。大量珊瑚中的珊瑚组织通常显示出变色并伴有炎症。大量Porites中粉红色色素沉着反应(PPR)的发生率,被描述为不同形状和大小的炎性粉红色病变,最近在全球范围内增加。据报道,FPs存在于PPR病变中,其中红色荧光蛋白(RFP)似乎在减少活性氧中起作用。然而,到目前为止,所涉及的色素的生化特征和可能的作用知之甚少。本研究旨在鉴定和表征在显示PPRs的大量Porites菌落中负责粉红色变色的蛋白质,以及评估颜料的差异分布和着色区域的抗氧化性能。
■使用凝胶过滤色谱法从PPR病变中提取CPs,并使用液相色谱-串联质谱法通过遗传分析进行鉴定。使用显微镜观察评估了珊瑚组织中CP和RFP的共存。测量了光合活性和过氧化氢清除活性,以评估珊瑚的胁迫条件。
■本研究表明,相同的CP(plut2。m8.16902.从块状Porites中分离出的m1)存在于PPR的粉红色斑点和斑块形态中。还发现CP与RFP共存于表现为PPR的珊瑚组织中,具有差异分布(息肉触须的coenosarc或尖端)。在受PPR影响的组织中发现了高过氧化氢清除率。
■CP和RFP的共存表明它们在珊瑚免疫中可能的不同作用。CP,它们在PPR病变中特别表达,可以作为受影响的珊瑚组织的抗氧化剂。总的来说,这项研究为我们理解CPs在珊瑚免疫中的作用提供了新的知识。
UNASSIGNED: Several fluorescent proteins (FPs) and chromoproteins (CPs) are present in anthozoans and play possible roles in photoprotection. Coral tissues in massive corals often display discoloration accompanied by inflammation. Incidences of the pink pigmentation response (PPR) in massive Porites, described as inflammatory pink lesions of different shapes and sizes, has recently increased worldwide. FPs are reported to be present in PPR lesions, wherein a red fluorescent protein (RFP) appears to play a role in reducing reactive oxygen species. However, to date, the biochemical characterization and possible roles of the pigments involved are poorly understood. The present study aimed to identify and characterize the proteins responsible for pink discoloration in massive Porites colonies displaying PPRs, as well as to assess the differential distribution of pigments and the antioxidant properties of pigmented areas.
UNASSIGNED: CPs were extracted from PPR lesions using gel-filtration chromatography and identified via genetic analysis using liquid chromatography-tandem mass spectrometry. The coexistence of CPs and RFP in coral tissues was assessed using microscopic observation. Photosynthetic antivity and hydrogen peroxide-scavenging activitiy were measured to assess coral stress conditions.
UNASSIGNED: The present study revealed that the same CP (plut2.m8.16902.m1) isolated from massive Porites was present in both the pink spot and patch morphologies of the PPR. CPs were also found to coexist with RFP in coral tissues that manifested a PPR, with a differential distribution (coenosarc or tip of polyps\' tentacles). High hydrogen peroxide-scavenging rates were found in tissues affected by PPR.
UNASSIGNED: The coexistence of CPs and RFP suggests their possible differential role in coral immunity. CPs, which are specifically expressed in PPR lesions, may serve as an antioxidant in the affected coral tissue. Overall, this study provides new knowledge to our understanding of the role of CPs in coral immunity.