Abstract：
Rift Valley fever virus is able to infect multiple organs and cell types, and the course of infection varies between viral strains and between individuals in particular according to age, genetic background, and physiological status. Studies on viral and host factors involve detecting and quantifying viral load at multiple time points and in multiple tissues. While this is classically performed by genome quantification or viral titration, in vivo imaging techniques using recombinant viruses expressing a bioluminescent or fluorescent protein allow noninvasive longitudinal studies on the same group of mice over the entire course of disease and the detection of unsuspected sites of infection. Here, we describe the protocol to monitor and characterize mouse infection with Rift Valley fever virus by in vivo imaging using recombinant viruses expressing light-emitting reporter genes.
摘要：
裂谷热病毒能够感染多种器官和细胞类型，感染过程在病毒株之间和个体之间变化，特别是根据年龄，遗传背景,和生理状态。对病毒和宿主因子的研究涉及在多个时间点和多个组织中检测和定量病毒载量。虽然这通常是通过基因组定量或病毒滴定进行的，使用表达生物发光或荧光蛋白的重组病毒的体内成像技术可以在整个疾病过程中对同一组小鼠进行非侵入性纵向研究，并检测未发现的感染部位。这里,我们描述了通过使用表达发光报告基因的重组病毒进行体内成像来监测和表征裂谷热病毒小鼠感染的方案。
