field guide

  • 文章类型: Journal Article
    基于长期且经常令人沮丧的经验,在世界各地收集了用于分类学和系统发育研究的劣质tape虫(Cestoda),考虑到获取新材料的障碍越来越多,一个简单的,提供易于使用和说明的方法指南(手册)。它主要集中在检查主机的关键步骤,从除弹枝外的多热脊椎动物中收集c虫,即,从射线鳍鱼(Actinopterygii),两栖动物和爬行动物(包括除鸟类以外的所有鼠类的近亲类),并将其固定用于后续的形态学和分子研究。建议应遵循以下方法学要点:(i)理想情况下仅应使用新鲜安乐死的宿主(未预先冷冻)进行寄生虫学检查;(ii)应通过照片记录检查的宿主;必要时还应保存宿主组织以供将来进行基因分型;(iii)应小心地分离,以保持头节完整并在固定之前进行适当清洁;(iv)一小片头菌,同时应将其固定在乙醇中,因为它们的DNA应始终使用热(加热)固定剂;这可以防止不自然的收缩或变形,并确保均匀的固定;(vi)每个样品(小瓶)都应适当标记(每个节食样品都应具有唯一的代码);(vii)应始终保存测序样本的凭证(同源或同源)以进行识别,并存入国际公认的收藏品。希望本指南可以帮助研究人员和学生正确处理有价值的cestodes材料,使其适合可靠的鉴定,包括基因分型和比较解剖学,这是任何后续生态的先决条件,生物地理学,系统发育生命周期或分子研究。
    Based on long-term and often frustrating experiences with the poor quality of tapeworms (Cestoda) collected throughout the world for taxonomic and phylogenetic studies, and considering the increasing obstacles to obtaining new material, a simple, easy-to-use and illustrated methodological guide (manual) is provided. It focusses mainly on key steps in examining hosts, collecting cestodes from poikilothermous vertebrates except elasmobranchs, i.e., from ray-finned fish (Actinopterygii), amphibians and \'reptiles\' (a paraphyletic group comprising all sauropsids except birds), and fixing them for subsequent morphological and molecular study. It is proposed that the following methodological points should be followed: (i) ideally only freshly euthanised hosts (not previously frozen) should be used for parasitological examination; (ii) hosts examined should be documented by photographs; host tissue should also be preserved for future genotyping if necessary; (iii) tapeworms should be detached carefully to keep the scolex intact and properly cleaned before fixation; (iv) a small piece of cestode tissue should be always preserved in molecular grade ethanol for DNA sequencing; (v) tapeworms should be fixed as quickly as possible after collecting them and while they are still alive, always using hot (heated) fixatives; this prevents unnatural contraction or deformation and ensures uniform fixation; (vi) each sample (vial) should be properly labelled (a unique code should be given to every cestode sample); (vii) vouchers of sequenced specimens (hologenophores or paragenophores) should always be preserved for identification, and deposited in internationally recognised collections. It is hoped that this guide helps researchers and students to properly process valuable material of cestodes to make it suitable for reliable identification including genotyping and comparative anatomy, which is a prerequisite for any subsequent ecological, biogeographical, phylogenetic life cycle or molecular study.
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  • 文章类型: Journal Article
    翻转的教室被教师感知甚至实践的方式有时是近似的。例如,虽然新冠肺炎疫情推动许多大学采用远程学习,在这种情况下,翻转教室经常被称为解决方案。这种诱因使翻转教室和远程学习之间存在混淆,这可能对学生和老师有害。此外,对于新来的老师来说,开始一种新的教学实践,如翻转课堂,可能是令人生畏和耗时的。由于这些原因,本文旨在分享一些实施翻转课堂的技巧,生物学和生物化学的例子。根据我们的经验,也根据当前的科学文献,我们围绕三个阶段构建这些建议:准备,实施,和后续行动。在准备阶段,我们建议尽早计划在课堂上和课堂外倒转时间,但也要明确地说,以及识别(或可选地创建)资源供学生自主学习。在实施阶段,我们建议(i)明确获取知识并培养学生的自主性;(ii)在课堂上探索主动学习;(iii)发展合作和分享技能;(iv)区分教学实践以适应学生的需求。最后,在后续阶段,我们建议(i)评估学生的学习和教学环境;(ii)照顾后勤和老师的姿势;(iii)记录翻转的教室,(四)分享教学经验。
    The way flipped classrooms are perceived and even practiced by teachers is sometimes approximate. For instance, while the Covid-19 pandemic has pushed many universities to adopt distance learning, flipped classrooms have often been mentioned as a solution in that context. This inducement maintains a confusion between flipped classrooms and distance learning that might be detrimental for students and teachers. Moreover, embarking on a new pedagogical practice such as flipped classroom could be intimidating and time-consuming for the newcomer teacher. For these reasons, this article aims to share some tips for implementing a flipped classroom, with examples from biology and biochemistry. Based on our experiences but also on the current scientific literature, we structured these advise around three phases: preparation, implementation, and follow-up. In the preparation phase, we advise planning early to invert time in class and outside the classroom, but also to say it explicitly, as well as to identify (or optionally create) resources for students to learn in autonomy. In the implementation phase, we suggest to (i) be explicit in the acquisition of knowledge and foster students\' autonomy; (ii) explore active learning in class; (iii) develop cooperation and sharing skills; and (iv) differentiate teaching practices to adapt to student needs. Lastly, in the follow-up phase, we propose to (i) evaluate both student learning and the pedagogical setting; (ii) take care of the logistics and the teacher\'s posture; (iii) document the flipped classroom, and (iv) share the teaching experience.
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