虎杖在民间医学中用作抗癌和抗风湿剂。本研究旨在研究抗血管生成,抗肿瘤,以及不同溶剂提取物和分离的皂苷的细胞毒性潜力。使用GC分析样品,气相色谱-质谱法(GC-MS)鉴定主要和生物活性化合物。包括甲醇提取物在内的植物样品的抗血管生成定量(Ph.Cr),其后续馏分;正己烷(Ph.十六进制),氯仿(Ph.Chf),乙酸乙酯(Ph.EtAc),正丁醇(Ph.Bt),水性(Ph.Aq),皂苷(Ph.Sp)使用鸡胚绒毛尿囊膜(CAM)测定进行。对含有肿瘤诱导质粒的根癌农杆菌进行马铃薯盘抗肿瘤试验。在接触毒性和MTT细胞活力测定后,对卤虫和小鼠胚胎成纤维细胞NIH/3T3细胞系进行了细胞毒性,分别。的GC-MS分析。Cr,Ph.十六进制,Ph.Chf,Ph.Bt,和Ph。EtAc鉴定了126、124、153、131和164种化合物,分别。在抗血管生成试验中,Ph.Chf,Ph.SP,Ph.EtAc,和Ph。Cr表现出最高的活性,IC50为28.65、19.21、88.75和461.53μg/ml,分别。在抗肿瘤试验中,Ph.SP,Ph.Chf,Ph.EtAc,和Ph。Cr最有效,IC50为18.39、73.81、217.19和342.53μg/ml,分别。在MTT细胞活力测定中,Ph.Chf,Ph.EtAc,Ph.Sp最活跃,引起79.00、72.50和71.50%的细胞毒性,分别,1000μg/ml,LD50为140、160和175μg/ml,分别。在整体研究中,Ph.Chf和Ph.Sp已显示出压倒性的结果,这表明它们作为癌症治疗剂来源的潜力。
Polygonum hydropiper is used as anti-cancer and anti-rheumatic agent in folk medicine. This study was designed to investigate the anti-angiogenic, anti-tumor, and cytotoxic potentials of different solvent extracts and isolated saponins. Samples were analyzed using GC, Gas Chromatography-Mass Spectrometry (GC-MS) to identify major and bioactive compounds. Quantitation of antiangiogenesis for the plant\'s samples including methanolic extract (Ph.Cr), its subsequent fractions; n-hexane (Ph.Hex), chloroform (Ph.Chf), ethyl acetate (Ph.EtAc), n-Butanol (Ph.Bt), aqueous (Ph.Aq), saponins (Ph.Sp) were performed using the chick embryo chorioallantoic membrane (CAM) assay. Potato disc anti-tumor assay was performed on Agrobacterium tumefaciens containing tumor inducing plasmid. Cytotoxicity was performed against Artemia salina and mouse embryonic fibroblast NIH/3T3 cell line following contact toxicity and MTT cells viability assays, respectively. The GC-MS analysis of Ph.Cr, Ph.Hex, Ph.Chf, Ph.Bt, and Ph.EtAc identified 126, 124, 153, 131, and 164 compounds, respectively. In anti-angiogenic assay, Ph.Chf, Ph.Sp, Ph.EtAc, and Ph.Cr exhibited highest activity with IC50 of 28.65, 19.21, 88.75, and 461.53 μg/ml, respectively. In anti-tumor assay, Ph.Sp, Ph.Chf, Ph.EtAc, and Ph.Cr were most potent with IC50 of 18.39, 73.81, 217.19, and 342.53 μg/ml, respectively. In MTT cells viability assay, Ph.Chf, Ph.EtAc, Ph.Sp were most active causing 79.00, 72.50, and 71.50% cytotoxicity, respectively, at 1000 μg/ml with the LD50 of 140, 160, and 175 μg/ml, respectively. In overall study, Ph.Chf and Ph.Sp have shown overwhelming results which signifies their potentials as sources of therapeutic agents against cancer.