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Abstract：
Introduction Collagen plays a vital role in maintaining the structural integrity of dentin, and its modification with bioactive compounds can enhance its mechanical properties and bonding capabilities. Aim This study aimed to evaluate the genotoxic effects of grape seed extract (GSE) and marine collagen peptide (MCP) on dental pulp-derived primary cells. Methodology Human dental pulp stem cells were isolated, cultivated, and then treated with GSE and marine collagen peptides. DNA fragmentation was assessed using DAPI (4\',6-diamidino-2-phenylindole) staining. Statistical analysis was performed using SPSS version 20 (IBM Corp., Armonk, NY, USA). Results The results showed that GSE exhibited a minimum level of cell death compared to marine collagen peptides. The viable cell count increased steadily over three days in all groups, with the control group showing the highest number of viable cells. The differences in viable cell count among the groups were statistically significant. Conclusion This study suggests that GSE and marine collagen peptides are highly biocompatible with dental pulp cells and could be considered for further clinical studies.
摘要：
引言胶原蛋白在维持牙本质的结构完整性中起着至关重要的作用，用生物活性化合物对其进行修饰可以增强其机械性能和键合能力。目的本研究旨在评估葡萄籽提取物（GSE）和海洋胶原肽（MCP）对牙髓来源的原代细胞的遗传毒性作用。方法分离人牙髓干细胞，栽培,然后用GSE和海洋胶原蛋白肽处理。使用DAPI（4'，6-二氨基-2-苯基吲哚）染色。使用SPSS第20版(IBMCorp.,Armonk,NY,美国）。结果结果显示,与海洋胶原肽相比,GSE表现出最低水平的细胞死亡。所有组的活细胞计数在三天内稳步增加，对照组显示最高数量的活细胞。各组之间的活细胞计数差异具有统计学意义。结论该研究表明，GSE和海洋胶原肽与牙髓细胞具有高度的生物相容性，可考虑进一步的临床研究。
